口服不同剂量胆碱在肉鸡血液中的代谢动力学研究  

Different-Dose Pharmacokinetic Study of Choline Chloride in Broiler by Oral Administration

作　　者：王斯佳[1] 蔡辉益[1] 刘国华[1] 闫海洁[1] 

机构地区：[1]中国农业科学院饲料研究所,家禽营养与饲料研究室,北京100081

出　　处：《动物营养学报》2015年第6期1899-1907,共9页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家肉鸡产业技术体系建设专项(nycyts-42-G4-03)

摘　　要：本试验旨在采用药代动力学的研究手段,探索胆碱在肉鸡血液中的吸收代谢规律。选用12只9周龄的爱拔益加（ AA）肉公鸡,随机分为3组,每组4只鸡,按体重分别单次口服氯化胆碱200和400 mg/kg（以胆碱计算）,并设空白对照组。采取血样,对血样中的胆碱浓度进行离子色谱法的测定,并用药代动力学处理软件WinNonlin 5.2采用非房室模型计算药代动力学参数。结果显示,空白对照组内源胆碱的合成水平基本稳定；不同给药剂量的氯化胆碱在肉鸡血液中的药代动力学过程存在差异,表观分布容积（ Vz/F）和血药表观清除率（ CL/F）差异极显著（ P〈0.01）；给药剂量在200~400 mg/kg,胆碱的药代动力学行为呈线性关系。肉鸡口服2种剂量的主要药代动力学参数如下。200 mg剂量组：达峰时间（Tmax）为8.0 h,达峰浓度（Cmax）为399.88 mg/kg,半衰期（ t1/2）为7.60 h,血浆浓度第一时间曲线下面积（ AUMC ）为5979.06 mg/（kg·h）,Vz/F为876.99 L/kg,CL/F为83.27 mg/（kg·h）；400 mg剂量组：Tmax为8.0 h, Cmax为445.88 mg/kg, t1/2为9.91 h, AUMC 为10899.78 mg/（ kg · h ）, Vz/F 为318.43 L/kg,CL/F为21.22 mg/（ kg·h）。200 mg剂量的胆碱更易入胞用于物质的生物合成。以上结果提示,单纯依靠增加氯化胆碱剂量无法实现提高胆碱生物利用率的目的。This experiment was conducted to compare the pharmacokinetics of choline chloride in broiler after oral administration at different doses. Twelve 9-week-old Arbor Acres male broilers were randomly divided into 3 groups with 4 birds in each group, and fed choline at 0, 200 and 400 mg/kg （ on basis of choline chloride） , respectively. The blood sample of each experimental bird was collected at 1, 2, 3, 4, 8, 12, 24, 36 and 48 h, respectively, and the serum choline concentration was analyzed by ion chromatography. The pharmaco-kinetics parameters were calculated by WinNonlin 5.2 using non-compartment model. The results showed that the serum choline concentration in birds fed no exogenous choline was stable during the 48 h. The pharmacoki-netics of choline chloride at different doses was significantly different, for both Vz/F and CL/F （P〈0.01）, with their regression between the serum choline concentration and time being linear respectively. The Tmax, Cmax, t1/2, AUMC, Vz/F and CL/F were 8. 0 h, 399. 88 mg/kg, 7. 60 h, 5 979. 06 mg/（ kg · h）, 876.99 L/kg and 83. 27 mg/（ kg · h ） respectively for the dose of 200 mg/kg group, and 8. 0 h, 445.88 mg/kg, 9.91 h, 1 0899.78 mg/（ kg·h） , 318.43 L/kg, 21.22 mg/（ kg·h） respectively for the the dose of 400 mg/kg group, with the choline at 200 mg/kg being more bioavailable. In conclusion, only in-creasing of choline chloride dosage can not achieve the purpose of improving the rate of choline bioavailability.

关 键 词：氯化胆碱 药代动力学 离子色谱法 肉鸡 

分 类 号：S831[农业科学—畜牧学]