脂多糖所致脓毒症诱导的急性肺损伤肺组织T淋巴细胞活化及共刺激分子表达的研究  

Activation of T lymphocytes and its expression of co-stimulatory molecules in acute lung injury induced by lipopolysaccharide caused sepsis

作　　者：林涛[1,2] 孔雅娴[1,2] 贾蓓[1,2] 刘三海 胡小玲[4] 曾辉[1,2] 

机构地区：[1]首都医科大学附属北京地坛医院传染病研究所,北京100015 [2]新发突发传染病研究北京市重点实验室,北京100015 [3]北京中医药大学基础医学院,北京100029 [4]首都医科大学附属北京同仁医院呼吸科,北京100730

出　　处：《中华实验和临床感染病杂志（电子版）》2015年第2期116-119,共4页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

基　　金：国家自然科学基金(No.81372095)

摘　　要：目的研究腹腔注射脂多糖(LPS)诱导的小鼠急性肺损伤(ALI)模型肺组织T淋巴细胞活化状态。方法健康雄性C57BL/6随机分为生理盐水(NS)腹腔注射组,LPS腹腔注射组,每组4只。模型建立后3 h,获取两组小鼠肺组织细胞并进行T淋巴细胞各亚群及活化指标染色,采用流式细胞术检测。结果与对照组(NS)相比,LPS腹腔注射组小鼠肺组织内抑制性共刺激分子PD-1、CD40/CD40L、CTLA-4在CD4^+和CD8^+T细胞表达水平均显著升高(P<0.05),协同共刺激分子CD28(MFI:298.50±4.44)表达水平降低;与对照组相比,早期活化分子CD69在LPS诱导的急性肺损伤模型小鼠肺组织内CD4^+T(MFI:848.30±95.57;t=6.8670,P<0.001)和CD8^+T淋巴细胞的表达水平显著升高(MFI:606.00±95.54;t=4.8780,P<0.01);晚期活化分子CD38在CD4^+T细胞表达显著升高(MFI:69.38±2.86;t=4.1150,P<0.01),在CD8^+T细胞表达无明显升高。结论 LPS诱导的急性肺损伤能够导致肺组织内T淋巴细胞早期活化,并且能够上调其多种抑制性共刺激分子表达,提示T淋巴细胞可能在ALI中发挥重要作用。Objective To investigate the activation of T lymphocytes in mouse model of acute lung injury（ALI） which induced by intraperitoneal injection of LPS.Methods Male mices were randomly divided into two groups：nonnal saline（NS）-IP and lipopolysaccharide（LPS）-IP.The mices of NS-IP and LPS-IP groups were exposed to NS and LPS via intraperitoneal injection,respectively.The activation of T lymphocytes,which migrated into lung,was analyzed by flow cytometer at 3 h after NS-IP and LPS-IP,respectively.Results Compared with the control group,the ALI group expressed higher co-stimulatory molecules PD-1,CD40/CD40 L and CTLA-4 on both CD4^＋ and CD8^＋ T cells significantly（P 〈0.05）.Moreover,LPS administration caused a high expression of an early activation marker CD69 on CD4^＋ T（MFI：848.30 ± 95.57;t = 6.8670,P 〈0.001） and CD8^＋ T cells（MFI：606.00 ± 95.54;t = 4.8780,P 〈0.01）.In addition to CD69,another activation marker CD38 also had been highly expressed on CD4＋ T cells（MFI：69.38 ± 2.86;t = 4.1150,P 〈0.01）,while a significantly high expression can not be found on CD8^＋T cells.Conclusions In this ALI model,T cells could be activated in lung tissue,and the high expression of multiple inhibitor co-stimulatory molecules suggests T lymphocytes may play an important role in the pathological process of ALI.

关 键 词：脂多糖 急性肺损伤 共刺激分子 活化 

分 类 号：R563[医药卫生—呼吸系统]