巨磁阻微流体免疫传感器快速定量检测D-二聚体  

An Integrated Giant Magnetoresistance Microfluidic Immuno-Sensor for Rapid Detection and Quantification of D-dimer

作　　者：高宇哲[1,2] 张磊[1,2] 霍卫松 石西增 廉洁 高云华[1] 

机构地区：[1]中国科学院理化技术研究所光化学转换和功能材料重点实验室,北京100190 [2]中国科学院大学,北京100049 [3]东莞博识生物科技有限公司,东莞523808 [4]中国人民公安大学刑事科学技术系,北京100038

出　　处：《分析化学》2015年第6期802-807,共6页Chinese Journal of Analytical Chemistry

基　　金：国家科技支撑计划(No.2013BAI03B)资助~~

摘　　要：将巨磁阻（GMR）传感器集成在微流体通道中,以100 nm磁颗粒为信号探针,研制了可快速检测血栓标志物D-二聚体的免疫传感器。用GMR传感器在线检测免疫反应后被捕捉在芯片上的磁颗粒的信息,测定血浆中D-二聚体的含量。通过对反应条件的优化,建立了GMR微流体传感器检测血浆中D-二聚体的方法。本方法的线性检测范围为5~6500 ng/m L,检出限为5 ng/m L。批内相对标准偏差〈12%;批间相对标准偏差〈14%,具有良好的稳定性和重现性。本方法可在9 min内完成检测,临床血浆样本的测试结果与日本Sysmex公司的CA1500血凝仪测试结果一致,具有灵敏度高、检测时间短、检测结果准确等优点。A method for rapid detection and quantification of D-dimer as a biomarker of thrombosis was developed by utilizing giant magnetoresistance （GMR） sensor integrated with microfluidic technology and 100 nm magnetic particles （MPs） as signal probes. The MPs binding on the chip through specific immune reaction was measured by the GMR sensor and the concentration of D-dimer was determined at the same time. The linear detection range of this method was from 5 ng/mL to 6500 ng/mL, and the detection limit was 5 ng/mL. The intra-assay precision （RSD） was less than 12% and the inter-assay precision was less than 14%. The whole analysis process could be completed within 9 min, and a good linear correlation between the results of GMR biosensor and Sysmex CA1500 was obtained.; This method has the advantages of high sensitivity, fast detection and good accuracy.

关 键 词：巨磁阻效应 微流体免疫传感器 D-二聚体 磁颗粒 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置] R446.11[自动化与计算机技术—控制科学与工程]