精子DNA碎片率与形态学及辅助生殖结局的相关性分析研究  

Correlation Analysis and Research Between Sperm DNA Fragments and Morphology and Assisted Reproductive Outcomes

作　　者：瞿虎[1,2] 江春强 汪中扬[1] 操日亮[3] 张靖[2] 陈文秋[2] 马波[1] 

机构地区：[1]中山大学附属第六医院泌尿外科,广东广州510655 [2]中山大学附属第六医院生殖医学研究中心,广东广州510610 [3]广东省妇幼保健院小儿外科,广东广州510010

出　　处：《中国医药指南》2015年第13期1-3,共3页Guide of China Medicine

基　　金：广东省医学科研基金项目;项目编号A2011215

摘　　要：目的研究分析不育患者精子形态学、DNA碎片率相互关系及与辅助妊娠结局关系。方法采用染色质扩散(SCD,彗星实验)方法检测计算精子DNA碎片指数(DFI),WHO人类精液检查与处理实验室手册(5版)标准测定精子畸形率,分析探讨二者之间及与辅助妊娠结局的关系。结果畸精组行ICSI治疗入组207例,单次妊娠成功率41.55%,同期正常对照组220例成功率47.73%,差异有统计学意义;DFI>30%与DFI≤30%组成功率分别为39.09%和46.39%,有显著性差异。结论男性不育患者中精子DNA碎片(DFI)可作为独立的精液检测、生育力及辅助生殖结局评估指标。Objective To analyze and research the relation between DFI in male infertile patients and sperm morphological test and assisted reproductive outcomes. Methods DFI were tested by SCD assay and Sperm morphology was defined by WHO laboratory manual for the examination and processing of human semen（5th edition）. The relation of DFI and sperm morphological test and assisted reproductive outcomes was analyzed. Results Abnormal sperm group for ICSI treatment group of 207 cases, single pregnancy success rate of 41.55%, the same period in normal control group of 220 patients with success rate of 47.73%, the difference has statistical significance; DFI30% and DFI is less than or equal to 30% power were 39.09% and 46.39%, there was significant difference. Conclusion The DFI can be a solitary evaluation index for semen test in male infertile patients

关 键 词：精子 DNA碎片 形态学 辅助生殖 

分 类 号：R71[医药卫生—妇产科学]