苦豆子提取过程中氧化苦参碱与苦参碱、氧化槐果碱与槐果碱的相互转化  

Study on Transformation Between Matrine and Oxymatrine, Oxysophocarpine and Sophocarpine in the Extraction Process of Sophora Alopecuroides

作　　者：冷晓红[1] 郭鸿雁[1] 陈海燕[1] 

机构地区：[1]宁夏职业技术学院,银川750021

出　　处：《中国现代应用药学》2015年第6期688-691,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：科技部国家科技支撑项目(2011BAI05B00)

摘　　要：目的研究苦豆子药用植物提取过程中氧化苦参碱与苦参碱、氧化槐果碱与槐果碱的相互转化。方法采用不同提取溶媒(水及55%乙醇)、不同p H及不同提取时间3个因素,HPLC测定苦豆籽、苦豆草植物中氧化苦参碱与苦参碱,氧化槐果碱与槐果碱的含量。结果随着提取时间的增加,氧化型生物碱转化为还原型生物碱的量随之增大;提取溶剂对苦豆子中生物碱转化的影响无明显差异;随着提取时间的增加,氧化苦参碱与苦参碱总量呈缓慢下降的趋势,氧化槐果碱与槐果碱总量也呈缓慢下降的趋势。结论氧化型生物碱与还原型生物碱转化趋势可为苦豆子生物碱质量控制提供依据,也可为工业化提取苦豆子生产工艺提供依据。OBJECTIVE To study the transformation between oxymatrine and matrine, oxysophocarpine and sophocarpine in the extraction process of Sophora alopecuroides. METHODS Different extraction solvent （water and 55% ethanol）, pH and extraction time were used to study the transformation rule of oxymatrine and matrine, oxysophocarpine and sophocarpine in Sophora alopecuroides Semen and Sophora alopecuroides Herba by HPLC. RESULTS With the increasing of extraction time, the amount of oxidized alkaloids transformed into reduced alkaloids increased; extraction solvent made no difference in the effect of the transformation of Sophora alopecuroides alkaloids; with the increasing of extraction time, the additive amount of oxymatrine and matrine decreased slowly, the additive amount of oxysophocarpine and sophocarpine also decreased slowly. CONCLUSION The results provide a basis for quality control of alkaloids of Sophora alopecuroides, also can provide a basis for industrial extraction of Sophora alopecuroides.

关 键 词：苦参碱 氧化苦参碱 槐果碱 氧化槐果碱 相互转化 

分 类 号：R284.2[医药卫生—中药学]