HPLC-ELSD测定银杏叶多糖组成及单糖含量  

Determination of Polysaccharides Composition and Monosaccharide Content in Ginkgo Folium by HPLC-ELSD

作　　者：任红[1] 乔洪翔 方玲 王如伟[1,4] 

机构地区：[1]浙江中医药大学药学院,杭州310053 [2]云南康恩贝植物研究院,昆明650217 [3]浙江省中药制药技术重点实验室,杭州310051 [4]浙江现代中药与天然药物研究院有限公司,杭州310051

出　　处：《中国现代应用药学》2015年第6期711-714,共4页Chinese Journal of Modern Applied Pharmacy

基　　金："重大新药创制"科技重大专项(2011ZX09203-001-18);浙江省科技计划项目(2009E10009)

摘　　要：目的 建立高效液相色谱法测定银杏叶多糖组成及单糖含量。方法 采用Agilent Zorbax Carbohydrate色谱柱（250 mm×4.6 mm,5μm）,乙腈-水（80∶20）为流动相,流速：1.0 mL.min 1,柱温：30℃。结果 色谱峰分离良好,鼠李糖在4.160～20.80μg、阿拉伯糖在8.040～40.20μg、果糖在4.096～20.48μg、甘露糖在4.000～20.00μg、葡萄糖在8.152～40.76μg、半乳糖在3.960～19.80μg内呈良好的线性关系。平均加样回收率鼠李糖为99.6%、阿拉伯糖为100.9%、果糖为99.7%、甘露糖为99.1%、葡萄糖为98.8%、半乳糖为100.1%。结论 该方法简单快速、稳定可行,可用于银杏叶中多糖成分质量控制。OBJECTIVE To establish an HPLC method for determination of polysaccharides composition and the monosaccharide content from Ginkgo Folium. METHODS The analysis was performed on Agilent Zorbax Carbohydrate（250 mm×4.6 mm, 5 μm） chromatographic column, and the mobile phase was acetonitrile-water（80∶20）, the flow rate was 1.0 m L·min-1 and the column temperature was maintained at 30 ℃. RESULTS The calibration curves were linear within the range of 4.160-20.80 μg for rhamnose, 8.040-40.20 μg for arabian sugar, 4.096-20.48 μg for fructose, 4.000-20.00 μg for mannose, 8.152-40.76 μg for glucose and 3.96-19.8 μg for galactose respectively. The recoveries of rhamnose, arabian sugar, fructose, mannose, glucose, galactose were 99.6%, 100.9%, 99.7%, 99.1%, 98.8%, 100.1%, respectively. CONCLUSION This method is simple and rapid, stability and feasible, it can be used for the quality control of polysaccharide compositions in Ginkgo biloba leaves.

关 键 词：银杏叶 多糖 单糖 含量测定 质量控制 

分 类 号：R917.101[医药卫生—药物分析学]