TOLL样受体在新生儿感染性疾病中的表达分析  

作　　者：徐颂周[1] 彭华[1] 胡晓艳[1] 赵方[1] 周于新[1] 

机构地区：[1]北京大学深圳医院儿科,广东518036

出　　处：《当代医学》2015年第19期1-3,共3页Contemporary Medicine

基　　金：2009年深圳市科技计划项目(200903083)

摘　　要：目的 观察Toll样受体1、2、3、4在新生儿感染性疾病中的表达情况,探讨它们在新生儿抗感染免疫中的作用。方法 收集感染性疾病患儿外周血（感染性疾病包含有败血症、细菌性肺炎、脑膜炎、坏死性小肠结肠炎、尿路感染）和爱婴区健康新生儿对照组外周血。采用RT-PCR法测定TLR 1-4 m RNA转录水平（与h GAPDH拷贝数比较）。结果 （1）TLR 1、2、4 m RNA在感染组表达明显增加,与对照组比较差异有统计学意义（P﹤0.05）;（2）TLR 3 m RNA在感染组表达较正常对照却明显下调,比较差异有统计学意义（P﹤0.05）。结论 与TLR 2、TLR 4一样,TLR 1和TLR 3也参与了新生儿感染性疾病的调控,但具体信号传导途径尚需进一步研究。Objective To observe the expression of Toll sample receptors 1, 2, 3, 4 in neonatal infectious diseases and to explore their roles in neonatal anti-infectious immunity.Methods The Peripheral Blood （PB） of the infant patients with infectious diseases （infectious diseases include sepsis, bacterial pneumonia, meningitis, necrotizing enterocolitis and urinary tract infection） and the PB of the control group of healthy newborns at the baby loving area had been collected. RT-PCR method had been used to determine the transcriptional level of TLR 1-4 mRNA （compared to hGAPDH copy number）.Results （1） The expression of TLR 1, 2, 4 mRNA was significantly increased in the infected group, and was significantly different from that in the control group （P〈0.05）;（2） The expression of TLR 3 mRNA in the infected group was relatively normal, which however was significantly lowered in the control group. There was statistically significant difference between the two groups （P〈0.05）.Conclusion Just like TLR 2 and TLR 4, TLR 1 and TLR 3 also participate in the regulation and control of neonatal infectious diseases, but the specific signal transduction pathways still need further study.

关 键 词：TOLL样受体 新生儿 感染 

分 类 号：R722.1[医药卫生—儿科]