钙黄绿素自组装膜制备及其对汞离子测定  

Preparation of calcein self-assembled multilayer film and interfacial recognition for mercury ion

作　　者：胡文英[1] 黄汉杰 黄建辉[1] 

机构地区：[1]莆田学院环境与生物工程学院,莆田351100 [2]莆田市计量所,莆田351100

出　　处：《化工新型材料》2015年第6期124-126,共3页New Chemical Materials

基　　金：国家自然科学基金资助项目(21103095);莆田市科技项目(2014S03(1));福建省质量技术监督局科技项目(FJQI2014010)

摘　　要：以石英基片作为基底采用硅烷化方法,通过Si-O键组装上γ-氨基丙基三乙氧基硅烷（KH550）,再利用吸附作用力将壳聚糖组装为第二层,最后将荧光试剂钙黄绿素通过与壳聚糖的酯化反应间接组装在石英基底表面,构建新型钙黄绿素自组装膜。结合荧光分析法将具有荧光特性的钙黄绿素自组装膜对汞离子检测。实验结果表明所构建的钙黄绿素自组装膜对浓度在0~3.00×10-6 mg/L汞离子具有线性关系,检出限为3.25×10-8 mg/L,钙黄绿素自组装膜法检出限低、灵敏度高、反应速度快、稳定性好,并应用此方法进行了水样中汞的检测。Based on the function of Si-O bond,theγ-aminopropyl triethoxysilane（KH550）was assembled on the quartz surface as the base using the silane method.Then chitosan as the second layer was assembled by adsorption force,and finally fluorescent reagent calcein was assembled on the surface of the quartz substrate by the indirect esterification between calcein and chitosan,the new calcein self-assembled monolayer film was build.the film was used to measure mercury ions withfluorescence analysis method.The results showed that the film had a linear relationship with mercury ions within the range of 0~3.00×10-6 mg/L.And the detected limit was 3.25×10-8 mg/L.Besides,The film had low detection limit,high sensitivity,fast response and good stability,and this method was also applied to measure the mercury ion in the water samples.

关 键 词：钙黄绿素 自组装膜 分子荧光 汞离子 

分 类 号：O657.3[理学—分析化学] TB383.2[理学—化学]