淀粉酶产色链霉菌1628发酵培养基及发酵条件的优化  

Optimization of medium and fermentation conditions of Streptomyces diastatochromogenes

作　　者：李丹婷[1] 袁小枫 申屠旭萍[1] 俞晓平[1] 

机构地区：[1]中国计量学院生命科学学院,浙江省生物计量及检验检疫技术重点实验室,浙江杭州310018

出　　处：《中国计量学院学报》2015年第1期99-104,共6页Journal of China Jiliang University

基　　金：国家自然科学基金资助项目(No.31401793);浙江省自然科学基金资助项目(No.LY12C14012);浙江省公益技术研究项目(No.2010C32063;2013C32008);杭州市科技计划项目(No.20120232B17)

摘　　要：淀粉酶产色链霉菌1628(Streptomyces diastatochromogenes)是一株具有广阔应用前景的生防菌株,其合成的主要活性物质为核苷类抗生素——丰加霉素.为了提高淀粉酶产色链霉菌的发酵水平,本研究采用单因素和正交实验设计,对菌株1628的发酵培养基和发酵条件进行了优化,优化后的最佳培养基配方为:可溶性淀粉1.5%、黄豆粉5%、FeSO40.1%、CaCO30.5%、NH4NO3 0.3%、KH2PO4 0.3%,最适初始pH7.0.在供试的发酵条件内,装液量60mL/250mL,发酵温度28℃,摇床转速180r/min,发酵时间为92h.活性物质丰加霉素的产量最高156.17mg·L-1,比优化前提高了3.3倍.Streptomyces diastatochromogenes 1628 has broad applications in biocontrol field and mainy biosynthesized uncleosides antibiotics-toyocamycin. In order to improve the toyocamycin production, single- factor experiments and orthogonal tests were designed to optimize the fermentation medium and conditions for Streptornyces diastatochromogenes 1628. The optimum fermentation medium was soluble starch 1.5%,soybean meal 5%, FeSO4 0.1%, CaCO3 0.5%, NH4NO3 0.3%, and KH2PO4 0.3%. The optimum cultural conditions were initial pH7.0, capacity 60 mL/250 mL, temperature 28℃,180r·min^-1 for 92 h. Under optimized conditions, the output of toyocamycin is 3.3 times higher than that of the control.

关 键 词：淀粉酶产色链霉菌 发酵优化 丰加霉素 单因素 正交设计 

分 类 号：R917[医药卫生—药物分析学]