几种微生态制剂对刺参幼参生长、存活和消化酶活性的影响  

Effects of several types of probiotics on growth,survival and digestive enzyme activity in juvenile sea cucumber Apostichopus japonicus

作　　者：郝佳[1,2] 吴英茜 刘晗奇 蔡明城 张赛赛[1] 李世陶[1] 王华[1] 王伟[1] 

机构地区：[1]大连海洋大学农业部北方海水增养殖重点实验室,辽宁大连116023 [2]大连市水产技术推广总站,辽宁大连116023 [3]长海县水产技术推广站,辽宁大连116500

出　　处：《大连海洋大学学报》2015年第3期248-252,共5页Journal of Dalian Ocean University

基　　金：大连市科学技术基金资助项目(2011J21DW011);国家自然科学基金资助项目(41101481);辽宁省高等学校杰出青年学者成长计划(LJQ2013080)

摘　　要：以体质量(2.00±0.52)g的刺参Apostichopus japonicus幼参为研究对象,定期将光合细菌、乳酸杆菌、芽孢杆菌、海洋红酵母4种微生态制剂及其四者的复合微生态制剂(按照终浓度比为4∶1∶4∶1的比例)添加到刺参幼参培育水体中,探讨其对刺参幼参生长、存活率和消化酶活性的影响。结果表明:养殖水体中添加不同的微生态制剂对刺参幼参的生长和存活有一定的促进作用,其中增重率和特定生长率最高出现在复合微生态制剂组,分别达到(125.97±8.26)%和(1.66±0.06)%/d,与对照组有显著性差异(P<0.05);添加微生态制剂能够显著提高刺参幼参的消化酶活性,尤其是添加将4种菌剂按适当比例复合的微生态制剂后,刺参幼参的消化酶活性与其他微生态制剂组相比有显著性差异(P<0.05)。研究表明,将4种有益菌按照一定比例复合后投放到幼参培育水体中相对于单个菌投放更能显著提高幼参消化酶的活性。Probiotics comprised of only photosynthetic bacteria, Lactobacillus, Bacillus, and oceanic red yeast or mixture of those microorganisms at a ratio of 4 ： 1 ： 4 ： 1 were regularly added into the tanks where sea cucumber Apostichopus japonicus with body weight of （2.00±0.52）g was cultured to evaluate the effect of several types of pro- biotics on growth, survival and digestive enzyme activity in juvenile sea cucumber. The results showed that addition of the probiotics led to improve significantly the growth rate and survival（ P〈0.05 ）, with the maximal weight gain （125.97±8.26） % and specific growth rate（1.66±0.06） %/d in the sea cucumber juveniles in the water supplemented with the mixture of the microorganisms, significantly higher than those in the juveniles in the control group （ P〈0. 05 ）. There were significantly higher digestive enzyme activities in the sea cucumber juveniles in the water supplemented with probiotics, especially supplemented with the mixture of the probiotics, than in the juveniles in the control group （P〈0. 05 ）.

关 键 词：刺参幼参 微生态制剂 生长 存活率 消化酶活性 

分 类 号：S917.1[农业科学—水产科学]