泰乐菌素高产菌株的筛选  

Screening of High Yield Strain for Tylosin

作　　者：张明明[1,2] 万丹[1,2] 徐玉崇 刘迎宾[1,2] 张小红[1] 

机构地区：[1]西北农林科技大学,陕西杨凌712100 [2]天方药业有限公司,河南驻马店463000

出　　处：《广州化工》2015年第12期87-89,共3页GuangZhou Chemical Industry

摘　　要：以弗氏链霉菌(Streptomyces fradiae)TP-116菌株作为出发菌株,采用紫外线、氯化锂和微波三重复合诱变,通过泰乐菌素抗性定向筛选,获得一株高产菌株YBT-06,并对其进行了遗传稳定性试验,结果表明突变株YBT-06生产代谢旺盛,遗传稳定性好,发酵效价比出发菌株提高了25%,达到11066μg/m L。Taking TP-116 strain of Streptomyces fradiae as a starting strain , using UV, lithium chloride and microwave triple compound mutagenesis , a high yield strainYBT-06 was got through the resistance of tylosin directional selection .The results on the genetic stability test showed that the mutant strain YBT -06 production metabolism exuberant , genetic stability was good , fermentation effect price had improved 25%than starting strains , reached 11066 μg/mL.

关 键 词：泰乐菌素 复合诱变 抗性筛选 

分 类 号：TQ[化学工程]