大鼠过敏性休克死后血清IgE、类胰蛋白酶的变化  

Changes of Serum IgE and Tryptase in Anaphylactic Shock Rats

作　　者：米丽[1] 高卫民[1] 杜中波[1] 曹志鹏[1] 张圆[1] 朱宝利[1] 

机构地区：[1]中国医科大学法医学院,辽宁沈阳110001

出　　处：《法医学杂志》2015年第3期181-184,共4页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(81273343)

摘　　要：目的观察大鼠过敏性休克死亡后血清IgE、类胰蛋白酶的变化规律,探讨其与死亡时间(PMI)、尸体及样本保存环境的相关性。方法建立大鼠过敏性休克死亡动物模型并分组,包括室温组、冷藏组、冷冻组、人工溶血组、血清样本保存组,并设立对照组。大鼠处死采血后,按离心后上层血清颜色进行溶血程度分级。通过ELISA法检测血清IgE、类胰蛋白酶在各组中的质量浓度。结果大鼠过敏性休克死亡后血清IgE、类胰蛋白酶水平明显高于对照组;室温、冷冻保存下血清IgE、类胰蛋白酶随PMI的不同有明显变化,冷藏保存下浓度相对稳定;随溶血程度的增加,血清IgE、类胰蛋白酶水平均呈升高趋势。样本不同温度条件下保存25 d,血清IgE、类胰蛋白酶无明显变化。结论大鼠过敏性休克死后血清IgE、类胰蛋白酶明显升高,但其水平受PMI、环境温度影响较大,尤其是室温或冷冻保存。Objective To explore the changes of serum IgE and tryptase caused by anaphylactic shock rats and discuss the relation to PMI and preservative environment of corpse and specimen. Methods Rats were used for establishing anaphylactic shock models and randomly divided into room temperature group, refrigeration group, frozen group, manual hemolysis group, specimen preservation group. And the control group was also established. The blood samples were collected after rats were sacrificed. The de- gree of hemolysis was graded according to the color of the upper layer of the serum. The mass concen- tration of IgE and tryptase in each group was detected by ELISA. Results The levels of serum IgE and tryptase in anaphylactic shock dead rats were higher than that of the control group. Room temperature and frozen made obviously differences on the levels of serum IgE and tryptase with various PMI. The levels of serum IgE and tryptase in refrigeration group showed relatively stable. The levels of serum tryptase and IgE were elevated with differently increasing hemolysis. The levels of serum IgE and tryptase showed no obvious changes during the specimen kept under different temperature conditions for 25 days. Conclusion Serum IgE and tryptase obviously increased in anaphylactic shock rats. However, the levels were influenced by PMI and environmental temperature, especially under the conditions of room temperature and frozen.

关 键 词：法医病理学 过敏反应 类胰蛋白酶类 免疫球蛋白E 大鼠 

分 类 号：DF795.1[医药卫生—法医学]