海洛因对大鼠海马、杏仁核和额叶皮质DLG4的影响  

Effect of Heroin on DLG4 Expression in Hippocampus, Amygdala and Frontal Cortex of Rats

作　　者：罗良鸣[1,2] 龚群[3] 刘建锋[1] 赵明权[1] 陈冬冬[4] 谢耀耀 朱华[2] 

机构地区：[1]温州市公安局刑事科学技术研究所,浙江温州325000 [2]温州医科大学法医学系,浙江温州325035 [3]浙江省公安厅刑侦总队,浙江杭州310009 [4]温州市公安局开发区分局刑侦大队,浙江温州325000 [5]温州市公安局龙湾区分局刑侦大队,浙江温州325000

出　　处：《法医学杂志》2015年第3期185-187,199,共4页Journal of Forensic Medicine

基　　金：浙江省公安厅重大科研资助项目(20110204)

摘　　要：目的检测不同时程海洛因依赖大鼠海马、杏仁核和额叶皮质discs大同源物4(discs large homolog 4,DLG4)的蛋白表达,探讨海洛因依赖对突触后致密结构的影响。方法采用腹腔内递增注射海洛因的方法,建立海洛因依赖的大鼠模型,用免疫组织化学法检测海洛因依赖9、18和36 d大鼠海马、杏仁核和额叶皮质DLG4蛋白的表达,并与对照组进行比较。结果随海洛因依赖时间的延长,海马、杏仁核和额叶皮质DLG4蛋白的表达逐渐降低。结论海洛因依赖可影响海马、杏仁核和额叶皮质突触后致密结构,并随依赖时间的延长,影响更加明显。Objective To observe the expression of discs large homolog 4 （DLG4） protein in hippocam- pus, amygdala and frontal cortex of rats and evaluate postsynaptic density in heroin dependence. Meth- ods The rat heroin dependent model was established by increasing intraperitoneal injection of heroin. DLG4 proteins in hippocampus, amygdala and frontal cortex of heroin dependent 9, 18, 36 days rats were detected with immunohistochemical staining and compared with that in the control group. Results DLG4 proteins in hippocampus, amygdala and frontal cortex were gradually reduced with extension of heroin dependent time. Conclusion Heroin dependence can affect postsynaptic density of hippocampus, amygdala and frontal cortex. The changes become more apparent with extension of heroin dependence time.

关 键 词：法医病理学 海洛因依赖 海马 杏仁核 额叶皮质 DLG4 大鼠 

分 类 号：DF795.1[医药卫生—法医学]