BAT在非离子型造影剂过敏反应中的诊断价值  

Diagnostic Significance of BAT in Anaphylaxis to Non-ionic Contrast Media

作　　者：张皓月[1] 许素军[2] 唐笑先[3] 牛记军[1] 郭相杰[1] 高彩荣[1] 

机构地区：[1]山西医科大学法医学院,山西太原030001 [2]太原市公安局小店分局,山西太原030002 [3]山西省人民医院CT室,山西太原030012

出　　处：《法医学杂志》2015年第3期188-190,共3页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(81172905)

摘　　要：目的通过测定免疫正常人、β-内酰胺类药物过敏者以及非离子型造影剂过敏者血液中CD63、肥大细胞羧肽酶A3(MC-CPA3)、人末端补体复合物SC5b-9,探讨嗜碱性粒细胞活化试验(basophil activation test,BAT)在非离子型造影剂过敏反应中的诊断价值。方法采用流式细胞术测定全血中嗜碱性粒细胞CD63表达率。采用ELISA法测定血清中MC-CPA3、血浆中SC5b-9含量。结果非离子型造影剂和β-内酰胺类药物过敏者血液中嗜碱性粒细胞CD63表达率、MC-CPA3以及SC5b-9含量均较免疫正常人升高(P<0.05)。结论非离子型造影剂过敏者血液中嗜碱性粒细胞和肥大细胞发生活化,补体系统的激活也参与了非离子型造影剂过敏反应的发生。流式细胞术分析BAT可作为非离子型造影剂过敏反应的诊断方法。Objective To investigate the diagnostic significance of basophil activation test （BAT） in ana- phylaxis to non-ionic contrast media through testing the content of CD63, mast cell-carboxypeptidase A3 （MC-CPA3）, and terminal complement complex SC5b-9 of the individuals by testing their levels in the normal immune group and the anaphylaxis groups to β-lactam drugs and non-ionic contrast media. Methods The CD63 expression of basophilic granulocyte in blood was detected by flow cytometry. The levels of MC-CPA3 in blood serum and SC5b-9 in blood plasma were detected by ELISA. Results The CD63 expression of basophilic granulocyte in blood, the levels of MC-CPA3 and SC5b-9 of anaphylaxis to non-ionic contrast media and β-1actam drugs were significantly higher than that in normal immune group （P〈0.05）. Conclusion There is activation of basophilic granulocytes, mast cells and complement system in anaphylaxis to non-ionic contrast media. BAT can be used to diagnose the anaphylaxis to non-ionic contrast media.

关 键 词：法医病理学 过敏反应 非离子型造影剂 嗜碱性粒细胞活化试验 CD63 肥大细胞羧肽酶A3 SC5B-9 

分 类 号：DF795.1[医药卫生—法医学]