GnRHR-Ⅱ在人类子宫内膜的表达  

Expression of GnRHR-Ⅱ in Human Endometria

作　　者：肖楠[1] 赵华[1] 殷莉[1] 靳镭[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院生殖医学中心,武汉430030

出　　处：《华中科技大学学报（医学版）》2015年第3期306-309,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：华中科技大学自主创新基金资助项目(No.2014ZHYX008)

摘　　要：目的探讨促性腺激素释放激素Ⅱ型受体(GnRHR-Ⅱ)在子宫内膜的分布及表达变化规律,分析GnRHR-Ⅱ与子宫内膜容受性的关系。方法通过诊断性刮宫技术,获取106名因"输卵管因素不孕"入院行宫腹腔镜手术的正常月经周期患者的子宫内膜组织,其中增生期形态子宫内膜54例,分泌期形态子宫内膜52例。采用免疫组化SP染色法,分别检测增生期、分泌期内膜基质细胞及腺上皮中GnRHR-Ⅱ的表达。根据表达强度及阳性率进行量化评分(HSCORE),采用SPSS 17.0统计分析软件对各组差异进行独立样本t检验。结果无论增生期还是分泌期,子宫内膜上均可见GnRHR-Ⅱ表达。增生期内膜基质细胞GnRHR-Ⅱ表达量明显高于腺上皮,差异有统计学意义(P<0.01)。分泌期腺上皮GnRHR-Ⅱ表达量明显高于基质细胞,差异有统计学意义(P<0.01)。增生期内膜基质细胞GnRHR-Ⅱ表达量明显高于分泌期内膜基质细胞,差异有统计学意义(P<0.01)。分泌期腺上皮GnRHR-Ⅱ表达量明显高于增生期腺上皮,差异有统计学意义(P<0.01)。结论 GnRHR-Ⅱ的分布及表达变化规律与子宫内膜为适应胚胎着床发生的形态、生理生化改变相一致,证实在一定范围内GnRHR-Ⅱ与子宫内膜对胚胎的容受性密切相关。Objective To investigate the distribution and expression variation of gonadotropin-releasing hormone receptor-Ⅱ（GnRHR-Ⅱ）in human endometria,and to evaluate the relationship between GnRHR-Ⅱand endometrial receptivity.Methods Endometrial tissues were obtained by diagnostic curettage from 106 patients who had normal menstration cycle and underwent hysteroscopy and laparoscopy due to tubal factor infertility.There were 54 cases of proliferative endometria and 52 cases of secretory endometria.The expression of GnRHR-Ⅱin proliferative endometrial stromal cells,proliferative endometrial glandular epithelia,secretory endometrial stromal cells and secretory glanduar epithelia was detected by immunohistochemical staining（SP method）.The results were quantitatively scored according to the intensity and positive rate of the expression.Independent samples t-test was carried out by using SPSS 17.0. Results GnRHR-Ⅱ was expressed in endometrial tissues in both proliferative phase and secretory phase.During the proliferative phase,GnRHR-Ⅱ was mainly distributed in stromal cells.The expression of GnRHR-Ⅱin stromal cells was significantly higher than that in glandular cells in the proliferative phase with difference being statistically significant（P〈0.01）.In the secretory phase,GnRHR-Ⅱ was mainly distributed in the glandular epithelia.The expression of GnRHR-Ⅱin glandular cells was significantly higher than in that of stromal cells.The difference was statistically significant（P〈0.01）.Endometrial tissues had a significantly higher expression of GnRHR-Ⅱin stromal cells in proliferative phase than in secretory phase（P〈0.01）.By contrast,GnRHR-Ⅱ was profoundly increased in glandular cells in secretory phase when compared with those in proliferative phase.Conclusion The change pattern of the distribution and expression of GnRHR-Ⅱ was consistent with the changes of morphology,physiology and biochemistry of the endometrial tissues to fit embryo implantation,which suggested that GnRHR-Ⅱis c

关 键 词：促性腺激素释放激素Ⅱ型受体 子宫内膜增生期 子宫内膜分泌期 内膜基质细胞 内膜腺上皮 子宫内膜容受性 

分 类 号：R711.6[医药卫生—妇产科学]