高效液相色谱串联质谱法研究环磷酰胺线性微透析探针体内外校正影响因素  

Influence factors of microdialysis linear probe recovery for in vivo and in vitro cyclophosphamide calibration by HPLC-MS/MS

作　　者：刘春生[1] 刘德鼎[2] 姜云云[3] 周莉[1] 王丹[1] 

机构地区：[1]第二炮兵总医院,北京100088 [2]解放军153医院,郑州450000 [3]解放军第101医院,无锡200433

出　　处：《国际药学研究杂志》2015年第3期362-364,共3页Journal of International Pharmaceutical Research

基　　金：国家自然科学基金青年项目资助(81301662)

摘　　要：目的建立环磷酰胺线性探针体内外回收率最优校正方法。方法以复方氯化钠注射液为灌注液,反向透析校正方法中的增量法与减量法校正线性探针,考察不同灌注流速对环磷酰胺线性探针回收率的影响。结果灌注流速为1.0μl/min时,环磷酰胺回收率大幅提高,增量法与减量法回收率分别由(24.11±1.75)%、(26.19±0.76)%提升至(50.93±0.91)%、(53.31±1.23)%,体内回收率为(26.19±0.76)%。结论环磷酰胺适宜进行微透析技术的取样,本实验校正方法简便有效。Objective To establish an optimal linear probe recovery correction method for in vivo and in vitro cyclophosphamide. Methods with ringer solution as the perfusion, retrodialysis gain and loss method were used to calibrate the linear probe. The impact of different perfusion flow rates on microdialysis relative recovery for cyclophosphamide was investigated by HPLC-MS/MS. Results When the perfusion was 1.0μl/min, in vitro recovery results were in the range of （24.11±1.75）%to （50.93±0.91）%for the gain method, and（26.19±0.76）% to（53.31±1.23）% for the loss method;recovery results in vivo were（26.19±0.76）%. Conclusion Cyclophosphamide is suitable for microdialysis sampling. This experiment calibration method is simple and effective.

关 键 词：微透析 环磷酰胺 线性探针 校正 高效液相色谱串联质谱法 

分 类 号：R927.11[医药卫生—药学] O657.63[理学—分析化学]