丙泊酚对大鼠脑缺血再灌注损伤的保护作用及其Caspase-3表达干预机制的研究  

Protection of propofol on cerebral ischemia reperfusion injury in rats and its mechanism of intervention for Caspase-3

作　　者：乔霖[1] 赵薇[1] 王新生[2] 邢珍[2] 

机构地区：[1]河北北方学院,河北张家口075000 [2]河北北方学院附属第一医院,河北张家口075000

出　　处：《现代药物与临床》2015年第6期629-632,共4页Drugs & Clinic

基　　金：河北省自然科学基金资助项目(H2012405016);河北北方学院自然科学研究计划项目(120176)

摘　　要：目的通过观察ip丙泊酚对脑缺血再灌注大鼠海马区神经细胞的影响,探讨丙泊酚对临床围手术期预防脑缺血再灌注损伤的作用。方法 SD大鼠60只随机分为丙泊酚组、假手术组和模型组。采用大脑中动脉线栓法制作左侧大脑动脉栓塞模型。在缺血再灌注前10 min时,丙泊酚组ip丙泊酚50 mg/kg,模型组ip给予等量生理盐水。假手术组只进行颈部切开、缝合操作而不进行缺血再灌注实验。缺血再灌注24 h采集脑组织。TTC染色法测定大鼠左侧脑组织梗死面积,免疫组化检测大鼠脑组织海马区Caspase-3的表达,原位杂交检测大鼠海马组织Caspase-3 m RNA的转录水平。结果与模型组比较,丙泊酚组脑组织灰白色区域明显缩小,改善了脑组织梗死面积,海马区Caspase-3的阳性表达和Caspase-3 m RNA的转录水平明显降低(P<0.01)。结论丙泊酚干预对大鼠脑缺血再灌注损伤有保护作用,其机制可能通过干预Caspase-3相关的细胞凋亡信号传导途径来完成。Objective To observe the effects of propofol ip injection on hippocampus nervous after cerebral ischemia-reperfusion injury, to study the prevention of propofol to cerebral ischemia-reperfusion injury during clinical peroperative period.Methods Sixty SD rats were randomly divided into propofol group, Sham group, and model group. Left cerebral arterial embolism models were made. At 10 min before cerebral ischemia-reperfusion, rats in propofol group were ip administered propofol （50 mg/kg）, and physiological saline was given to Sham group. Rats in model group were done the same operation except cerebral ischemia-reperfusion and propofol. At 24 h after ischemia reperfusion, the tissues were collected. The areas of left cerebral infarct of rats were measured by TTC staining method, Caspase-3 express in hippocampus was measured by immunohistochemical methods, and transcriptional levels of Caspase-3 mRNA were detected by in situ hybridization test.Results Compared with model group, gray area obviously shrank and incanus areas were improved in propofol group. The expression of Caspase-3 and transcriptional levels of Caspase-3 mRNA in hippocampus of rats in propofol group were lower than those in model group, with significant differences between two groups （P〈0.01）. Conclusion Propofol intervention before ischemia-reperfusion maybe prevent ischemia reperfusion injury, and the mechanism is related to the apoptosis signal transduction pathway through Caspase-3 mediation.

关 键 词：丙泊酚 脑缺血再灌注 CASPASE-3 凋亡 

分 类 号：R965[医药卫生—药理学] R972[医药卫生—药学]