cspB异源表达对提高转基因烟草植株耐高温能力的影响  

Ectopic Expression of cspB Confers Resistance to High Temperature in Tobacco( Nicotiana tabacuum)

作　　者：徐萍莉[1] 夏人杰 何道一[1] 

机构地区：[1]淮北师范大学生命科学学院/资源植物生物学安徽省重点实验室,安徽淮北235000

出　　处：《核农学报》2015年第6期1068-1073,共6页Journal of Nuclear Agricultural Sciences

基　　金：安徽省自然科学研究重点项目(KJ2011A249)

摘　　要：为了解枯草芽孢杆菌cspB基因在植物中异源表达对植物应对逆境的影响,将cspB基因插入到pBI121的35S启动子下游取代原有的gus A基因,该基因的植物表达载体被命名为p BI121-csp B,通过农杆菌介导的转化法转化烟草,通过筛选培养获得转基因植株。RT-PCR分析表明,转基因烟草株系中均有csp B基因表达,其中TN012株系中表达量较大。转基因植株TN012和非转基因对照植株经38℃高温处理一周,再在25℃条件下恢复生长,经过7d的恢复生长,TN012的生物量明显高于对照植株。高温处理前,TN012与对照植株光合速率无明显差异,高温处理后TN012的光合速率显著高于对照植株的。TN012细胞中的可溶性蛋白在高温处理后明显上升,而对照植株的则明显下降。结果表明csp B基因植物异源表达提高植物对高温的抗性,cspB基因可以作为用于耐高温作物育种的基因资源。In order to understand effects of csp B on stress adaptation of plant,cspB was inserted in p BI121 under the control of Ca MV35 S promoter by replacing the gus A gene and the construct was designated as pBI121-cspB. Transgenic tobacco plants with csp B gene by Agrobacterium-mediated transformation were regenerated on selection medium. The results of RT-PCR analysis showed csp B expressed in all transgenic plants and high level of expression of csp B was detected in the transgenic plant TN012. The transgenic plant TN012 and control plant were treated under 38℃ for 1week and then grew under 25℃. The biomass of TN012 was greater than that of control plant after 7 d recovery growth..There was no significant difference between TN012 and control plant in the photosynthetic rate before high temperature treatment. However,the photosynthetic rate of TN012 was significant higher than that of control plant after high temperature treatment. Moreover,after high temperature treatment,total protein content was significantly increased in TN012,but was significantly decreased in control plant. The results indicated that ectopic expression of csp B confers resistance to high temperature in plants,and csp B is one of genes beneficial to crop breeding in terms of high temperature resistance.

关 键 词：cspB基因 转基因植株 高温抗性 烟草 

分 类 号：S572[农业科学—烟草工业]