白芍对牙周炎小鼠血清IgG的调节和治疗效果研究  

Effect of Aqueous Extracts of Radix Paeoniae Alba on the Level of Serum IgG in an Experimental Mouse with Periodontitis

作　　者：宋宁[1] 邝亦元[1] 廖志清[1] 周铸民[1] 丁桂聪[1] 黄世光[2] 

机构地区：[1]广东省深圳市儿童医院口腔科,深圳518026 [2]暨南大学医学院口腔医学系,广东广州510632

出　　处：《实用中西医结合临床》2015年第4期1-3,共3页Practical Clinical Journal of Integrated Traditional Chinese and Western Medicine

基　　金：广东省深圳市卫生计生系统科研项目(编号:201401048)

摘　　要：目的:观察白芍水提取物对治疗牙周炎小鼠血清Ig G水平的调节作用及其治疗小鼠牙周炎的效果。方法:将48只清洁级昆明小鼠按随机数字表法分为三组:正常对照组;实验性牙周炎组:参照Kimura等方法建立小鼠实验性牙周炎模型,并从建模后第5周起用蒸馏水灌胃;白芍治疗组:同上法建立牙周炎模型,从第5周起用白芍水提取物灌胃治疗。各组小鼠于建模后第4、6、8、10周分批处死,处死前测量牙龈指数,制作牙体牙周组织联合切片观察其组织病理学改变。采用摘眼球法取血液并分离血清,用ELISA法检测其血清Ig G水平的变化。结果:组织学观察:实验性牙周炎组可见大量炎性细胞浸润,牙槽骨进行性吸收,并见大量破骨细胞。与实验性牙周炎组比较,白芍治疗组牙周组织炎症程度明显减轻,在建模后第8、10周牙龈指数均明显降低(P<0.05)。实验性牙周炎组和白芍治疗组在建模后血清Ig G水平均明显高于正常对照组(P<0.01),实验性牙周炎组在建模后第10周血清Ig G水平明显低于第4周(P<0.05),白芍治疗组在建模后第6、8、10周血清Ig G水平均明显高于实验性牙周炎组(P<0.01)。结论:白芍水提取物能够提高小鼠实验性牙周炎的血清Ig G水平,对牙周炎有一定的治疗效果。Objective： To explore the effect aqueous extracts of Radix Paeoniae Alba on periodontitis mice and compare the influence on the level of serum IgG. Methods： 48 SPF 12-week-old male Kunming mice were selected and randomly divided into three groups： control group, experimental periodontitis group, and Radix Paeoniae Alba treatment group. The experimental mouse periodontitis model was induced with Kimura＇s method, the mice were gavaged with distilled water from the beginning of the 5th weeks. The mice in the Radix Paeoniae Alba treatment group were induced by the same method, and were gavaged with the aqueous extracts of Radix Paeoniae Alba. Four mice were sacrificed at the end of 4,6,8 and 10 weeks in each group, the Gingival Index （GI） of the mice had been measured before the mice were killed, the level of serum IgG was measured by ELISA. The histopathological changes of periodontal tissue were observed under microscope with haematoxylin-eosin staining. Results： A serious inflammatory cell infiltration, alveolar progressive absorption and a large number of osteoclasts were observed in the experimental periodontitis group. However, the inflammation in periodontal tissue was significantly decreased in Paeoniae Alba Radix treatment group. GI in Radix Paeoniae Alba treatment group was lower than that in experimental periodontitis group （P〈0.05）. The level of serum IgG in experimental periodontitis group and Radix Paeoniae Alba treatment group was significantly higher than that in control group （P〈0.01）, the level of sermn IgG in Radix Paeoniae Alba treatment group was significantly higher than that in experimental periodontitis group at 6, 8, 10 weeks （P〈0.01）, the level of serum IgG in Experimental periodontitis group at 10 week was significantly lower than that at 4 week. Conclusions： Aqueous extracts of Radix Paeoniae Alba have therapeutic effect on periodontitis mice, can improve the level of serum IgG.

关 键 词：牙周炎 白芍 IG G 小鼠 

分 类 号：R781.4[医药卫生—口腔医学]