红细胞渗透脆性试验方法改良的探讨  

Discussion of Improved Examination Method of Osmotic Fragility Test

作　　者：张玲[1] 胡朝晖[1] 潘建华[1] 吴鹏[1] 周锐雄 朱庆义[1] 

机构地区：[1]广州金域医学检验中心血液病理中心实验室,广州510330

出　　处：《现代检验医学杂志》2015年第3期94-97,共4页Journal of Modern Laboratory Medicine

摘　　要：目的：改良红细胞孵育渗透脆性试验（Osmotic fragility test，OFT）验证改良方法与标准操作方法结果一致性。方法对 OFT 采取加样取量及检测试剂减半和采用不同检测设备进行比色评价改良方法检测效果，即选取脆性增大和脆性降低样本100例（阳性50例，阴性50例），用一种加样改良和测试改良方法与传统方法进行结果比对。结果100例OFT 样本采取加样量与试剂减半检测出的结果与厂家标准操作加样量所测结果验证一致（T＝1．6608，P ＞0.05），两组数据间对比差别无统计学意义。结论OFT 改良方法替代传统 OFT 初筛方法可以更快、更客观地出结果服务于临床，且能有效地降低试剂和人力成本，提高检测效率。因此，该法可作为批量计生体检单位及检测设备缺乏的基层医院筛查珠蛋白生成障碍性贫血方法首选条件。Objective To verify result consistency of the improved examination method and the standard operation method with the erythrocyte osmotic fragility test （OFT）.Methods The samples and reagents were reduced half volume and used different testing equipment to evaluate methods for OFT.Selected 100 samples that the brittleness were increased and de-creased （50 samples positive and negative respectively）,used a kind of improved examination method and traditional test method to evaluate the consistency.Results The detection result of improved examination method and the detection results of the manufacturer standard method were consistent （t=1.660 8,P 〉0.05）.So,there was no significant difference of con-trast between two groups of data.Conclusion Improved mothed OFT alternatives to traditional OFTscreening method could be faster,more objectively the results of clinical service,and could effectively reduce reagent and manpower cost,improve the efficiency of work.Therefore,this method could be used to groups detection and lack of equipment for the primary care of hospital screening thalassemia desease method is preferred.

关 键 词：红细胞孵育渗透脆性试验 改良方法 检测设备 试剂成本 提高效率 

分 类 号：R446.11[医药卫生—诊断学]