珠海地区性病实验室淋球菌培养鉴定能力室间质评结果分析  

Analisis of External Quality Assessment of Neisseria Gonorrhoeae Identification and Validation in STD Laboratories of Zhuhai Area

作　　者：沈守星 刘小凤 徐刚 魏秋姣 

机构地区：[1]珠海市慢性病防治中心,广东珠海519000

出　　处：《现代检验医学杂志》2015年第3期159-161,共3页Journal of Modern Laboratory Medicine

基　　金：珠海市医学科研基金项目（项目编号：2015J026）.

摘　　要：目的：为了建立珠海地区淋球菌耐药检测网络，了解性病实验室淋球菌分离鉴定的能力，及时发现影响鉴定结果的因素和存在的问题。方法2011-2014年度淋球菌质控样品统一发放到参评实验室，各室按照常规操作进行淋球菌培养分离鉴定和 PPNG 实验，各实验室在规定时间内回报鉴定结果，由市慢性病防治中心对回报结果进行统计分析，并将考评结果反馈给各参评实验室。结果四年间共发放220份质评样本，综合考核成绩平均合格率88.6％（39／44）；淋球菌鉴定结果符合率分别为78.8％（26／33），90.3％（28／31），87.9％（29／33）和100％（33／33）；PPNG 符合率分别为80.8％（21／26），78.6％（22／28），86.2％（25／29）和100％（33／33）。结论通过规范化建设性病实验室，每年进行室间质评活动，珠海地区性病实验室淋球菌培养鉴定能力明显提高，但个别实验室存在人员粗心大意、结果报告准确性和规范性等问题，仍需加强实验室质量控制工作。Objective To recognize the ability to identify and validate N .gonorrhoeae in Zhuhai STD laboratories to timely find out the factors or problems influencing the results.Methods Samples were uniformly delivered to STD laboratories in 2011-2014.The laboratories identified and validated N .gonorrhoeae and PPNG.Test results were analyzed and evaluation results were fed back to participating laboratories.Results 220 samples were delivered to STD laboratories in four years. The average percentage of qualified labs was 88.6% （39/44）.The coincidence rates for N .gonorrhoeae quality control sam-ples respectively were 78.8% （26/33）,90.3% （28/31）,87.9% （29/33）and 100% （33/33）in 2011 - 2014.The coinci-dence rates for PPNG respectively were 80.8% （21/26）,78.6% （22/28）,86.2% （25/29）and 100% （33/33）.Conclusion Through the construction of STD standardization laboratory and the external quality assessment every year,the STD labora-tories of Zhuhai area improved the ability to validate and identify N .gonorrhoeae obviously.But several laboratories had some problem,such as careless technicians and inaccurate reports.So quality control should be still strengthened.

关 键 词：性病实验室 淋球菌 室间质评 

分 类 号：R378.16[医药卫生—病原生物学] R446[医药卫生—基础医学]