精索静脉曲张病人精子DNA损伤与精浆蛋白的表达  

SPERM DNA DAMAGE AND EXPRESSION OF SEMINAL PLASMA PROTEIN IN PATIENTS WITH VARICOCELE

作　　者：李勉洲 贾月峰[1] 曹廷帅 黄巍[1] 王沛涛[3] 王新生[3] 

机构地区：[1]青岛大学附属医院泌尿外科,山东青岛266003 [2]山东大学附属济南中心医院泌尿外科 [3]青岛大学附属医院男性科

出　　处：《青岛大学医学院学报》2015年第3期274-277,280,共5页Acta Academiae Medicinae Qingdao Universitatis

基　　金：青岛市科技发展计划资助项目(13-1-3-22-NSH)

摘　　要：目的 探讨精索静脉曲张（VC）不育病人精子DNA损伤与精浆蛋白表达的关系。方法 采用精子染色质扩散试验（SCD）方法,检测42例VC不育病人及20例正常生育男性（A组）的精子DNA完整性（DFI）,VC不育病人中精液分析异常者（B组）22例,精液分析正常者（C组）20例;应用蛋白质组学双向凝胶电泳、质谱技术检测各组精浆蛋白表达情况。结果 与A组比较,B组、C组精子密度、活力降低,DFI增高,差异有显著意义（F=31.38~785.17,q=4.21~55.99,P〈0.05）;与C组比较,B组精子密度、活力降低,DFI增高,差异有显著意义（q=6.77~28.87,P〈0.05）。与A组比较,B组、C组精子活动率降低,差异有显著性（H=45.79,Z=-5.47、-4.03,P〈0.01）;与C组比较,B组精子活动率降低,差异有显著性（Z=-5.14,P〈0.01）。3组表达差异的蛋白质点15个,8个蛋白质点得到成功鉴定,这些蛋白功能与精子活力及获能、免疫、DNA损伤、凋亡、分子运输相关,其中与DNA损伤相关蛋白SMG-1、IGFBP-3仅表达于B组。结论 VC导致的精子DNA损伤可能是引起男性不育的重要原因。Objective To explore the relationship between DNA damage and the expression of seminal plasma protein （SPP） in infertile men with varicocele. Methods Applying sperm chromatin dispersion （SCD） test, semen samples of 42 infer tile men with varicocele-22 with abnormal semen analysis （group B） and 20 with normal semen analysis （group C） and 20 men of normal fertility （group A） were detected for their sperm DNA integrity, and the expressions of SPP in each group were detected by two dimensional gel electrophoresis （2 DE） and mass spectrometry （MS）. Results Compared with group A, the sperm density and vitality in groups B and C decreased, and the sperm DNA integrity increased, the differences being significant （F=31.38-785.17,q=4.21-55.99,P〈0.05）, a comparison between groups C and B showed that sperm density and vitality in group B de clined, and sperm DNA integrity increased, the differences being significant （q = 6.77-28.87, P〈0.05）. Compared with group A, the sperm activity rate in groups B and C declined （H =45.79,P〈0.05）, a comparison between groups C and B showed a decrease of sperm activity rate in group B （Z=-5.47, 4.03;P〈0.01） ; compared with group C, the sperm viability of group B was signi fieantly lower （Z=-5.14,P〈0.01）. Fifteen protein spots of interest were submitted to mass spectrometry and eight sports su ccessfully identified, these proteins were associated with motility of sperm and capacitation, immune function, DNA damage, apoptosis and molecular transportation, of which, DNA damage-associated proteins-SMG-1 and 1GFBP-3-expressed only in group B. Conclusion Sperm DNA damage due to varicocele might be an important cause of male infertility.

关 键 词：精索静脉曲张 蛋白质组学 不育 男(雄)性 精子 DNA损伤 

分 类 号：R698.2[医药卫生—泌尿科学] R697.24[医药卫生—外科学]