冠心病病人血清Fetuin-A及IL-35浓度变化及应用评价  

SERUM LEVELS OF FETUIN-A AND IL-35 AND THEIR APPLICATION VALUE IN PATIENTS WITH CORONARY HEART DISEASE

作　　者：杨晓龙[1] 王元松[2] 柴建[3] 王清[4] 刘成玉[1] 

机构地区：[1]青岛大学医学院诊断学教研室,山东青岛266021 [2]青岛大学医学院临床技能学实验室,山东青岛266021 [3]青岛大学医学院 [4]青岛大学医学院附属医院检验科,山东青岛266021

出　　处：《青岛大学医学院学报》2015年第3期300-302,305,共4页Acta Academiae Medicinae Qingdao Universitatis

摘　　要：目的 探讨冠心病（CHD）病人血清胎球蛋白A（Fetuin-A）、白细胞介素-35（IL-35）浓度的变化及其应用价值,并分析其与α羟丁酸脱氢酶（HBDH）和乳酸脱氢酶（LDH）的相关性。方法 采用酶联免疫吸附法测定60例不同类型CHD病人血清Fetuin-A、IL-35浓度,同时检测CHD病人血清LDH、HBDH浓度,并与20例健康体检者（对照组）进行比较。结果 CHD组血清Fetuin-A、IL-35浓度显著低于对照组（t=3.60、3.34,P〈0.05）。急性心肌梗死（AMI）组和不稳定型心绞痛（UAP）组血清Fetuin-A、IL-35浓度显著低于稳定型心绞痛（SAP）组和对照组（F=4.28、8.77,q=3.54~77.28,P〈0.05）。CHD组血清HBDH、LDH浓度均高于对照组（t=2.21、2.20,P〈0.05）。AMI组血清HBDH、LDH浓度显著高于UAP组、SAP组及对照组（F=8.56、8.72,q=6.01~7.07,P〈0.01）。CHD病人血清Fetuin-A与IL-35浓度呈正相关（R^2=0.870,P〈0.01）;Fetuin-A与HBDH、LDH浓度均呈负相关（R^2=0.034、0.028,P〈0.1）。CHD组血清LDH、HBDH、Fetuin-A、IL-35浓度变化与对照组相比的AUCROC为0.602~0.746,AMI组为0.816~0.915,UAP组为0.544~0.815。结论 血清Fetuin-A和IL-35浓度变化对CHD有较高的诊断价值;在对不同类型CHD病人的诊断中,除IL-35对UAP病人价值较低外,两者均有较高的诊断价值。Objective To investigate the changes of serum Fetuin-A and interleukin-35 （IL-35） levels and their correla- tion with hydroxybutyrate dehydrogenase （HBDH） and lactic dehydrogenase （LDH） in patients with coronary heart disease （CHD）. Methods Employing enzymeqinked immune sorbent assay, serum Fetuin-A, and IL-35 were detected in 60 patients with different-type CHD, and, at the same time, serum LDH and HBDH were measured as well. The results were compared with 20 healthy controls. Results Serum levels of Fetuin-A and IL-35 in CHD patients were notably lower than that in the controls （t = 3.60,3.34 ; P 〈0.05）, that in acute myocardial infarction （AMI） and unstable angina pectoris （UAP） were lower than that stable angina pectoris （SAP） （F = 4.28,8.77 ; q = 3.54 - 77.28 ; P 〈 0.05 ）. Serum LDH and HBDH in CHD were higher than that in the control group （t = 2.21,2.20;P〈0.05） and that in AMI were higher than that in U AP, SAP and control groups （F ： 8.56, 8.72;q：6.01-7.07;P〈0.01）. In CHD, Fetuin-A was positively correlated with IL-35 （R2=0.870,P〈0.01）, and Fetuin-A was negatively correlated with both HBDH and LDH （R 2 ： 0.034,0.028;P d0.1）. Compared with the control group, the area under the receiver operating characteristic curves （AUC^ROC） of LDH, HBDH, Fetuin-A and IL-35 were 0. 602- 0. 746 in CHD patients; AUC^ROC of AMI patients were 0.816-0.915; AUC^ROC of UAP patients were 0.544--0.815. Conclusion The changes of serum levels of Fetuin-A and IL-35 have a greater value in the diagnosis of coronary heart disease, except IL-35 for unstable angina pectoris.

关 键 词：冠状动脉疾病 甲胎蛋白类 白细胞介素35 诊断 

分 类 号：R446.1[医药卫生—诊断学] R541.4[医药卫生—临床医学]