胆石六号汤剂对豚鼠胆色素结石模型胆囊的抗炎作用  

Anti-inflammatory effect of Danshiliuhao decoction on pigment stones of Guinea pigs

作　　者：刘龙忠[1] 钱英[1] 胡姗姗[2] 陈泽慧[1] 田应彪[1] 

机构地区：[1]遵义医学院附属医院药剂科,贵州遵义563003 [2]遵义医学院药学院,贵州遵义563003

出　　处：《中国临床药理学杂志》2015年第12期1162-1164,共3页The Chinese Journal of Clinical Pharmacology

基　　金：贵州省教育厅自然科学基金资助项目[黔教合K字(2012)054号];贵州省科学技术基金资助项目[黔科合J字(2008)215号]

摘　　要：目的探究胆石六号汤剂对胆色素结石豚鼠胆囊肿瘤坏死因子-α(TNF-α)及核转录因子(NF-κB)表达的影响。方法将雌性豚鼠40只随机分成3组,空白组10只,模型组15只,实验组15只。空白组除外,其余2组均采用饮食诱导法,建立胆色素结石模型。实验组,灌胃胆石六号汤剂7.7g·kg-1·d-1,其余2组均灌胃用同体积的0.9%氯化钠。30 d后,观察各组豚鼠成石率,用免疫组织化学技术检测胆囊TNF-α及NF-κB蛋白表达和胆囊病理组织的改变情况。结果模型组成石率为90.62%,明显高于空白组26.76%,表明造模成功;实验组成石率为11.00%,明显低于模型组90.62%,可见药物具有抗胆结石的作用。与空白组比较,模型组的TNF-α、NF-κB蛋白表达量明显增加(P<0.01)。与模型组比较,实验组的TNF-α、NF-κB蛋白表达量明显较少(P<0.05)。结论胆石六号汤剂可以明显减少胆色素结石豚鼠的成石率,这可能与药物降低胆色素结石豚鼠胆组织TNF-α、NF-κB蛋白的表达有关。Objective To study the effect of Danshiliuhao decoction on the expression of tumor necrosis factor -α（ TNF -α） and nuclear factor-κB（ NF -κB ） in the bile pigment with stone in Guinea pigs. Methods The female Guinea pigs were randomly divided into three groups.The blank group （ n =10 ） , the model group （ n =15 ） and test group （ Danshiliuhao,n=15 ） .The latter two groups of the Guinea pigs were both modeled as the bile pigment stones model by feeding them with a different diet .The Guinea pigs in test group were given Danshi-liuhao decoction 7.7 g· kg-1· d-1 for 30 days.The rate of stone formation and the expression of the gallbladder TNF-αand NF-κB were observed af-ter administration by immunohistochemistry.Results The rate of stone formation was 90.62% in model group, significantly higher than that （26.76%） in the blank group.The results suggested that the model for Danshiliuhao is successfully built and the drug is an effective agent for anti-gallstones.The expression of the TNF -αand NF -κB in test group were significantly lower than model group. The difference was statistically significant （ P〈0.05 ） .Conclusion Danshiliuhao Decoc-tion significantly reduced the rate of pigment stone formation in Guinea pigs.This may be related to the decreased expression of TNF-αand NF-κB proteins in gallbladders of Guinea pigs by the drug.

关 键 词：胆色素结石 胆石六号汤剂 肿瘤坏死因子-α 核转录因子 

分 类 号：R975.5[医药卫生—药品] R965.1[医药卫生—药学]