牙龈卟啉单胞菌感染加速牙龈上皮细胞细胞周期的机制  

The effect of Porphyromonas gingivalis on the proliferation in human gingival epithelial cells

作　　者：谭丽思[1] 潘春玲[1] 王宏岩[1] 赵戬[1] 赵海礁[1] 潘亚萍[1] 

机构地区：[1]中国医科大学口腔医学院牙周科,辽宁沈阳110002

出　　处：《中国微生态学杂志》2015年第6期677-680,共4页Chinese Journal of Microecology

基　　金：国家自然科学基金资助项目(81070834和81271153)

摘　　要：目的通过体外建立牙龈卟啉单胞菌(P.gingivalis)内化牙龈上皮细胞模型,探讨P.gingivalis W83、ATCC 33277菌株内化牙龈上皮细胞加速牙龈上皮细胞细胞周期的机制。方法 P.gingivalis W83、ATCC 33277菌株内化牙龈上皮细胞,实时定量PCR和蛋白印记方法检测细胞周期素D1和细胞周期素E1的表达。结果 MOI为100∶1,P.gingivalis ATCC 33277和W83菌株内化IHGE细胞Cyclin D1基因和蛋白在4 h时开始升高,6 h至12 h与对照组比较差异有统计学意义。Cyclin E1基因和蛋白在4 h、6 h时表达与对照组比较差异无统计学意义,在10 h和12 h Cyclin E蛋白表达水平明显高于对照组。P.gingivalis ATCC 33277与W83菌株内化IHGE细胞,两组间Cyclin D1、Cyclin E1基因和蛋白表达比较差异无统计学意义。结论 Cyclin D1和Cyclin E1 mRNA和蛋白的提前表达和高表达,诱导一系列细胞增殖基因的转录,缩短G1期进程。Objective To investigate the regulatory mechanism of P. gingivalis on the proliferation in human gingival epithelial cells through using an in vitro model. Methods The protein and mRNA of Cyclin D1 and Cyclin E1 were determined by Real-time quantitative RT-PCR and Western immunoblot in IHGE cells internalized with P.gingivalis W83 and ATCC 33277. Results We observed that no significant differences in Cyclin D1 mRNA and protein expression levels were measured at 4 h after invasion in infected and control cells（ P 〉0. 05）. The mRNA levels of Cyclin D1 were significantly upregulated when cells were treated with P. gingivalis from 6 h to 12 h after infection（ P〈0. 05）. Cyclin E1 mRNA and protein expression was increased up to a twofold induction observed when cells were treated with P. gingivalis at 10 h and 12 h. Conclusion We confirmed that P. gingivalis significantly enhances IHGE cell proliferation by overexpression of mRNA and protein of Cyclin D1 and Cyclin E1.

关 键 词：牙周炎 牙龈卟啉单胞菌 细胞周期 

分 类 号：R512.62[医药卫生—内科学]