金樱子多糖抗氧化作用的实验研究  

Experiment study on antioxidant capacity of Rosa laevigata Michx Polysaccharide in vitro

作　　者：韦玉兰[1] 苏上贵[2] 黄燕军[2] 张庆梅[1] 乐宁[1] 黄勇奇[2] 罗广秀 

机构地区：[1]广西中医药大学 [2]广西医科大学,520023

出　　处：《广西中医药》2015年第3期61-64,共4页Guangxi Journal of Traditional Chinese Medicine

基　　金：广西自然科学基金项目(编号:2011GXNSFA018272);广西教育厅科研项目(编号:200911LX233);广西中医药大学科研课题(编号:P2007003)

摘　　要：目的:探讨金樱子多糖体外抗氧化能力。方法:分别采用Smirnoff水杨酸法和邻苯三酚自氧化法观察金樱子多糖对羟自由基(·OH)和超氧阴离子(O2-·)的清除作用,同时观察了金樱子多糖对1,1-二苯基-2-三硝基苯肼(DPPH自由基)的清除作用和总还原能力。结果:金樱子多糖具有良好的抗氧化能力,并对·OH、O2-·及DPPH自由基具有较强的清除能力,但对不同的自由基敏感程度不同,对·OH和O2-·的清除能力在实验浓度范围内随浓度的增高而增强。结论:金樱子多糖具有良好的抗氧化活性,提示其具有良好的抗衰老作用。Objective： To explore the antioxidant capacity of Rosa laevigata Michx Polysaccharide in vitro. Method： The methods of Smirnoff salicylic acid and pyrogallol autoxidation were used to observe the scavenging effect of Rosa laevigata Michx Polysaccharide on hydroxyl free radical （· OH） and superoxide anion （O2·）. They were also used to observe the scavenging effect and total reducing power on 1,1-diphenyl-2 trinitrobenzenesulfonic hydrazine （DPPH radical）. Results：Rosalaevigata Michx Polysaccharide had good antioxidant activity and powerful scavenging capacity on · OH, O2· and DPPH radical, but had distinct sensitivity on different DPPH. The scavenging capacity on · OH and O2· improved as the concentration increased in the range of experimental concentration. Conclusion： Rosalaevigata Michx Polysaccharide has good antioxidant activity,which suggests its good anti-aging effect.

关 键 词：金樱子 多糖 抗氧化 自由基 

分 类 号：R285.5[医药卫生—中药学]