电纺壳聚糖与聚乙烯醇纤维膜的生物相容性研究  

Study on the biocompatibility of electrospun chitosan/poly(vinyl alcohol) fiber membrane

作　　者：杨静[1] 王永[2] 叶川[3] 张俊标 杨龙[1] 孙博[1] 刘琴[1] 马敏先[2] 

机构地区：[1]贵阳医学院,贵州贵阳550004 [2]贵阳医学院附属口腔医院,贵州贵阳550004 [3]贵阳医学院附属医院骨科,贵州贵阳550004

出　　处：《海南医学》2015年第12期1719-1721,共3页Hainan Medical Journal

基　　金：贵州省科技厅联合基金(编号:M2012-22)

摘　　要：目的制备壳聚糖与聚乙烯醇纤维膜并研究其体外的生物相容性,为应用于临床提供一定的理论依据。方法利用静电纺丝技术制备壳聚糖与聚乙烯醇纤维膜,通过扫描电子显微镜对其超微结构进行表征,采用溶血实验和细胞毒性实验评价其体外生物相容性。结果通过电纺制备的壳聚糖与聚乙烯醇纤维膜,质地均匀,伴有少量的珠状结构,纤维直径在60-300 nm之间,溶血实验显示溶血率为2.80%,无溶血作用,细胞毒性实验中,纤维膜的细胞增殖度为89.96%,细胞毒性为1级,评分合格,无细胞毒性。结论通过静电纺丝法可成功制备壳聚糖与聚乙烯醇纤维膜,生物相容性较好,有望应用于口腔临床中。Objective To prepare of chitosan/poly（vinyl alcohol） fiber membrane and to explore the biocom-patibility in vitro, in order to provide certain theoretical basis for clinical application. Methods The chitosan/poly（vi-nyl alcohol） fiber membrane was prepared by electrospinning technique. Its ultrastructure was characterized by scan-ning electron microscope （SEM）, and its in vitro biocompatibility was studied with hemolysis test and cytotoxicity test. Results The chitosan/poly （vinyl alcohol） fiber membrane prepared was found to have a good appearance and homoge-nous fibers, with a little beads and the diameter of 60-300 nm in average. The hemolysis rate was 2.8%, which means no hemolysis. The cell relative growth rate of the fiber membrane was 89.86%, with the cytotoxicity of gradeⅠ, which indi-cated no cytotoxicity. Conclusion The chitosan/poly （vinyl alcohol） fiber membrane can be prepared by the electrospin-ning technique successfully, with good biocompatibility, which is expected to be applied in oral clinical practice.

关 键 词：静电纺丝 壳聚糖 聚乙烯醇 生物相容性 

分 类 号：R318[医药卫生—生物医学工程]