有氧运动抑制高血压大鼠肠系膜动脉BKCa通道蛋白亚基表达上调  

Aerobic Exercise Inhibits Protein Expression Level of BKCa Channel in Mesenteric Arteries from Spontaneously Hypertensive Rats

作　　者：张琳[1] 张寒梦 叶芳[1] 石丽君[1] 

机构地区：[1]北京体育大学运动生理教研室,北京100084

出　　处：《天津体育学院学报》2015年第1期42-46,65,共6页Journal of Tianjin University of Sport

基　　金：国家自然科学基金项目(项目编号:31371201);北京市自然科学基金项目(项目编号:5132017);新世纪优秀人才支持计划项目(项目编号:NCET-11-0850)

摘　　要：目的：探究有氧运动对高血压大鼠（SHR）肠系膜动脉血管平滑肌细胞大电导钙激活钾（BKCa）通道α和β1亚基蛋白表达及血管舒缩性的影响.方法：选用12周龄雄性自发性高血压大鼠（SHR,n=24）以及正常血压对照组（WKY,n=12）.将SHR随机分组,高血压安静对照组（SHR,n=12）和高血压有氧运动组（SHR-EX,n=12）,各组大鼠先进行1周适应性训练,之后SHR-EX组进行为期8周的跑台训练.9周后,取各组大鼠的肠系膜动脉（2～3级）,进行HE染色,离体微血管张力测定及蛋白免疫印迹分析.结果：（1）SHR组肠系膜动脉的管壁厚度明显高于WKY组（P＜0.01）,但SHR-EX组肠系膜动脉的管壁厚度明显低于SHR组（P＜0.01）;（2）Iberiotoxin（10-7M）作为BKCa通道选择性阻断剂可诱发各组血管张力增加,增高幅度为SHR＞SHR-EX＞WKY,NS11021作为BKCa通道选择性激动剂可使血管张力下降,下降幅度为SHR＞SHE-EX＞WKY;（3）SHR组BKCa通道的α和β1亚基表达上调,且以β1亚基上调更为显著,SHR-EX组的两亚基上调减弱,且以β1亚基变化更为显著.结论：高血压诱导大鼠肠系膜动脉BKCa通道功能增强,负反馈调节血管张力增加,但有氧运动可显著抑制高血压伴随的大鼠肠系膜动脉BKCa通道的α和β1亚基表达上调,从而改善血管功能.Objective： The present study was designed to investigate the effects of chronic exercise on the molecular expression of α- and [31-subunit of large-conductance Ca2＋-activated K＋ （BKCa） channels and vascular contractility in rat mesenteric arterial smooth muscle cells from spontaneously hyperten- sive rats. Methods： Three-month-old male normotensive Wistar-Kyoto rats （WKY, n=12） and SHRs （n=24） were used. SHRs （n=24） were separated into a sedentary group （SHR） and an exercise group （SHR-EX） at random （n=12 in each group）. After a 1-week acclimation period, rats in SHR-EX were sub- jected to aerobic excrelse on a motor-driven treadmill. After 8 weeks, HE staining was used to analyze the vascular morphology change. Mesenteric arterial tensions were measured in vitro. Immunohistochemistry and Western blots assay were also conducted. Results： 1 ） Compared with WKY, the systolic blood pressure and pulse pressure were significantly increased with hypertension （P〈0.01 ）. However, they significantly dropped in SHR-EX compared with its sed- entary group （P〈O.01）. 2） The wall thickness of mesenteric artery in hypertension group was obviously increased, but it effectively decreased in SHR-EX compared with SHR. Selective BKCa channel blocker （Iberiotoxin10-7M） induced a marked increase of vascular tension in MAs in all three groups. Howev- er, these effects were greatly increased in SHR group. 3） Selective BKCa channel activator （NS11021） induced concentration-dependent vasorelaxation in MAs precontracted with norepinephrine. However, these effects were greatly decreased in SHR group. 4） Western blotting showed that the protein expression of BKCa ct- and β-subunit was significantly increased with hypertension, and the increase of β1 subunits was larger than that of α subunits, whereas aero- bic exercise could effectively ameliorate the changes. Conclusions ： Hypertension is associated with an increase of BKCa channel protein expression, which is a negativ

关 键 词：有氧运动 肠系膜动脉 BKCA通道 血管张力 蛋白表达 

分 类 号：G804.5[文化科学—运动人体科学]