富血小板纤维蛋白凝胶析出液对人牙髓细胞体外矿化的影响  

The effect of platelet-rich fibrin gel precipitate liquid on mineralization of human dental pulp cells in vitro

作　　者：何璇[1] 韦维[1] 陈文霞[1] 

机构地区：[1]广西医科大学附属口腔医院牙体牙髓病科,广西南宁530021

出　　处：《右江民族医学院学报》2015年第3期349-351,共3页Journal of Youjiang Medical University for Nationalities

基　　金：国家自然科学基金资助项目(81160133)

摘　　要：目的探索富血小板纤维蛋白(platelet-rich fibrin,PRF)凝胶析出液对人牙髓细胞(human dental pulp cells,hDPCs)体外矿化的影响。方法组织块法培养hDPCs。采用Choukroun一步离心法制备PRF凝胶。将新鲜制备的PRF凝胶浸泡于DMEM培养基中,于第7d取析出液。用PRF凝胶析出液孵育hDPCs 3d后更换矿化诱导液。采用茜素红染色和RT-PCR检测人牙髓细胞矿化的潜能。结果矿化诱导21d后,茜素红染色观察到实验组有少量钙结节生成,而对照组无钙结节生成;RT-PCR结果显示,实验组hDPCs碱性磷酸酶(ALP)的表达为对照组的1.5倍,差异具有统计学意义(P<0.05)。结论 PRF凝胶析出液可促进人牙髓细胞矿化。Objective This study was designed to investigate the effect of platelet-rich fibrin gel （PRF gel）precipitate liquid on the mineralization of human dental pulp cells （hDPCs）in vitro. Methods The hD-PCs were separated and cultured by using tissue block culture method.PRF gel was prepared by Choukroun＆#39;s protocols.The newly prepared PRF gel was dipped in DMEM culture media,the precipitate liquid of PRF gel was collected on day 7.hDPCs were treated with mineralization induction solution 3 days after being incubated with the precipitate liquid of PRF gel.The capacity of mineralization was measured by using alizarin red stai-ning and RT-PCR. Results Twenty-one days after mineralization induction,a small amount of mineral-ized nodules on alizarin red staining were observed in experimental group while no mineralized nodule was ob-served in control group;RT-PCR revealed that the expression of alkaline phosphatase （ALP）in experimental group was 1.5 times higher than that in control group,comparison yielded statistical difference （P 〈0.05）. Conclusion The precipitate liquid of PRF gel can accelerate the mineralization of hDPCs.

关 键 词：富血小板纤维蛋白凝胶 析出液 人牙髓细胞 矿化 

分 类 号：R322.41[医药卫生—人体解剖和组织胚胎学]