水稻稻曲病抗性鉴定技术及影响因子研究  

Resistance Identification and Influence Factor of Rice False Smut

作　　者：伏荣桃[1,2] 王剑[1,2] 卢代华[1,2] 张鸿[1,2] 龚学书[1,2] 陈雪娟[1,2] 任鸿志[1,2] 毛建辉[1,2] 

机构地区：[1]四川省农业科学院植物保护研究所,成都610066 [2]农业部西南作物有害生物综合治理重点实验室,成都610066

出　　处：《中国农学通报》2015年第18期266-272,共7页Chinese Agricultural Science Bulletin

基　　金：四川省科技厅支撑计划项目"水稻稻曲病轻简化防控关键技术研究与示范"(2013NZ0024);四川省财政基因工程项目"水稻稻曲病QTL分析"(2011JYGC06-021);四川省科技厅育种攻关项目"水稻抗病性鉴定及监测技术研究与服务"(2011NZ0098-1);四川省成都市科技支撑计划项目"水稻对稻曲病抗性机理与抗性资源筛选评价研究"(12DXYB203NC-002)

摘　　要：水稻稻曲病是一种水稻穗部病害，现已从次要病害上升为水稻的主要病害之一，为了提高水稻品种对稻曲病性抗性鉴定的准确性，本试验在田间和大棚条件下分别从接种方法、接种体、栽培时期、发病条件和环境条件等方面做了全面研究。对比3种接种方法发现，注射．喷雾相结合方法的发病效果最好；在4种接种体中，分生孢子一菌丝片段混合液接种发病最严重，菌丝片段和上年保存的厚垣孢子不能引起发病；在田间或大棚条件下病原菌接种后，进行遮阳和喷雾保湿处理对发病效果好，发现温度在25～30℃和相对湿度在85％以上更有利于稻曲病侵染和显现症状；试验结果还显示大棚条件下的鉴定效果稳定于田间条件。因此建议在进行抗病性鉴定时可在田间多点异地人工．自然诱发的抗性鉴定结果上，增加水稻品种大棚人工接种测定，这样可以获得更有效、更可靠的鉴定结果。Rice false smut is a kind ot panicle olsease, nowauays it has risen from a secondary disease to one of the main diseases of rice. To increase accuracy of resistance identification for rice false smut, this experiment conducted a comprehensive study, including inoculation methods, inoeula, planting time, infection condition and environmental condition in the field and greenhouse. This study compared three inoculation methods and found that the best effect was the combination of injection and spraying method. Among four inocula, the mixture of hypha fragment and thin-wall conidia caused the most severe disease, whereas mycelium fragments and chlamydospores kept in room last year could not cause false smut. The treatments of sun-shade and moisturizing spray had a better disease effect after inoculated with pathogen in field or greenhouse conditions. The temperature of 25-30℃ and relative humidity above 85% were more favorable to infection and symptom appearance of the disease. In addition, the results showed that more stable identification effect could be obtained in the greenhouse than in field condition. In order to get a more effective and reliable assessment

关 键 词：水稻 稻曲病 抗性鉴定 接种方法 

分 类 号：S432[农业科学—植物病理学]