洋葱细胞质雄性不育基因分子标记的开发  

Development of a Molecular Marker Identifying Cytoplasmic Male Sterility Gene in Onion( Allium cepa L. )

作　　者：李永博[1,2] 刘冰江[2] 霍雨猛[2] 缪军[2] 杨建平[1] 吴雄[2] 杨妍妍[2] 

机构地区：[1]山东农业大学园艺科学与工程学院,山东泰安271018 [2]山东省农业科学院蔬菜花卉研究所,山东济南250100

出　　处：《山东农业科学》2015年第6期1-4,共4页Shandong Agricultural Sciences

基　　金：国家自然科学基金项目(31301786;31201635)

摘　　要：根据细香葱orf A501开发的特异引物,以洋葱细胞质雄性不育系为试材进行PCR扩增,对获得的片段进行回收测序,根据测序结果设计引物进行染色体步移,获得部分侧翼序列,开发了鉴定洋葱细胞质基因型的SCAR标记,命名为OC2175。该标记在洋葱不育系中扩增出一条2 175 bp的特异条带和1 053 bp的条带,而保持系中仅扩增出1 053 bp的条带。对17组不同遗传背景的不育系及其相应的保持系进行验证,该SCAR标记的鉴定结果与田间表型判断结果完全吻合。The specific primers of orfA501 in chives and cytoplasmic male sterile line of onion were used to PCR amplification. The amplified fragment was extracted and sequenced. The primers were designed ac- cording to the obtained sequence for genome walking, and partial flanking sequence was obtained. Then a SCAR marker was developed and named as OC2175. With this marker, a special fragment of 2 175 bp could be obtained from the male sterile lines and a 1 053 bp fragment was amplified from both （s） -cytoplasm and （N） - cytoplasm. Seventeen pairs of male sterile lines and their corresponding maintainer lines with different genetic backgrounds were used to verify this SCAR marker. The results indicated that the SCAR marker was perfectly suitable for distinguishing the cytoplasm types S or N in onion.

关 键 词：洋葱 细胞质雄性不育 分子标记 

分 类 号：S633.203.6[农业科学—蔬菜学]