基质固相分散—气质联用法快速检测黄瓜中四类农药残留  

Simultaneous determination of four types of pesticide residues in cucumber by matrix solidphase dispersion-GC-MS

作　　者：张维一[1] 周颖[1] 都林娜[1] 王亮[1] 

机构地区：[1]温州科技职业学院,浙江温州325006

出　　处：《浙江农业学报》2015年第6期1000-1007,共8页Acta Agriculturae Zhejiangensis

基　　金：浙江省科技厅公益项目(2012C37020);温州市科技创新团队项目(C2012005)

摘　　要：基于基质固相分散（matrix solid-phase dispersion,MSPD）前处理方法和气相质谱联用技术,通过优化前处理以及气相质谱方法,建立了同时测定黄瓜中有机磷、有机氯、拟除虫菊酯和氨基甲酸酯4类常见农药多残留的方法。结果表明,50种农药在50~1 000μg·L-1范围内线性关系良好,相关系数为0.973 0~0.999 7,且大部分在0.99以上,各农药上机定量下限为1~2μg·kg-1。10和50μg·kg-1两个水平的50种农药加标回收试验表明,加标水平为50μg·kg-1时,农药回收率全部在70%~100%,10μg·kg-1水平时,有45种农药回收率在70%~100%,相对标准偏差为0.23%~15.42%。该方法灵敏度高,快速简单,准确可靠,适用于黄瓜中4类常见农药多残留的日常检测工作。Based on matrix solid-phase dispersion（ MSPD） pretreatment and gas chromatography-mass spectrometry（ GC-MS）,a method for simultaneous determination of four types of pesticide residues（ organophosphorus,organochlorine,pyrethroid and carbamate） in cucumber was developed by optimizing the parameters of pretreatment and GC-MS. Results indicated that the linear relation between peak area and concentration of the 50 pesticides in the range of 50 μg·L- 1to 1 000 μg·L- 1were good,and the correlation coefficients of most pesticides were higher than0. 99（ from 0. 973 0 to 0. 999 7）. The detection limits of 50 pesticides was 1- 2 μg·kg- 1. The recoveries of 50 pesticides ranged from 70% to 100% at the 50 μg·kg- 1spiking levels,while at the level of 10 μg·kg- 1,there were only 45 pesticides. Besides,the relative standard deviations（ RSDs） ranged from 0. 23% to 15. 42%. Thus,the research provided a sensitive,rapid,simple,and accurate analysis method for the simultaneous determination of four types of pesticide residues in cucumber. It was appropriate for the simultaneous identification and quantification of multi-residues in cucumber.

关 键 词：基质固相分散—气质联用 农药 黄瓜 

分 类 号：S482[农业科学—农药学] O658[农业科学—植物保护]