煎煮法提取卷叶贝母组培物总生物碱研究  

Study on Extracting Total Alkaloids from Fritillaria Cirrhosa D. Don Tissue Cultures by Decocting Method

作　　者：王跃华[1] 徐恩琴 郭翠平[1] 梅英[1] 刘涛[1] 王晓蓉 

机构地区：[1]成都大学生物工程学院,四川成都610016 [2]成都恩威集团(投资)有限公司,四川成都610041

出　　处：《成都大学学报（自然科学版）》2015年第2期108-110,共3页Journal of Chengdu University（Natural Science Edition）

基　　金：成都市科技局科技计划(12GGYB413SW-001)资助项目

摘　　要：采用煎煮法提取卷叶贝母组培物的总生物碱并测定其含量.通过对煎煮材料大小、浸泡时间和煎煮时间等主要因素进行实验,并以贝母素乙为测定标准,采用紫外分光光度法测定了不同提取条件下的总生物碱含量.实验结果显示,煎煮材料大小、浸泡时间和煎煮时间3个因素均影响卷叶贝母组培物中总生物碱含量.选择适宜的卷叶贝母组培物大小、浸泡时间和煎煮时间可以在一定程度上提高其有效物生物碱的溶出量.This paper extracts the total alkaloids from Ffitillaria cirrhosa D. Don tissue cultures by the decocting method and determines the content of total alkaloids. By researching main factors such as material size, soaking time and decoction time and taking Peiminine as determination standard, we adopt UV spectrophotometry to determine the content of total alkaloids under different extracting conditions. The experimental results show that the above three factors can all influence the content of total alkaloids. Therefore, the appropriate selection of tissue culture size, soaking time, and decoction time can enhance the extraction rate of the total alkaloids in some degree.

关 键 词：卷叶贝母 煎煮法 生物碱 

分 类 号：R242.2[医药卫生—中医临床基础] S567.231[医药卫生—中医学]