四君子汤对肠梗阻解除后肠黏膜屏障功能影响的实验研究  

Effects of early application of Sijunzi decoction on intestinal mucosal barrier after relieving obstruction

作　　者：李琳[1] 

机构地区：[1]天津市南开医院第一外科,天津300100

出　　处：《天津中医药》2015年第6期356-358,共3页Tianjin Journal of Traditional Chinese Medicine

摘　　要：[目的]利用可复性机械性完全性肠梗阻模型,观察肠梗阻解除后自然恢复及中药四君子汤对肠屏障功能的影响。[方法]选用健康大耳白兔,随机分为正常对照组、梗阻2 d组、自然恢复2 d组、中药治疗2 d组、自然恢复4 d组、中药治疗4 d组,每组6只。对动物模型梗阻解除后2 d、4 d时内毒素、肠黏膜损伤评分、小肠上皮层黏膜完整性情况分别进行检测和观察。[结果]结果表明在肠梗阻解除后,内毒素水平逐渐降低(P<0.05),且中药治疗组血浆内毒素水平以及Chiu肠黏膜损伤评分明显低于自然恢复组(P<0.05),中药治疗组绒毛表面积明显高于自然恢复组(P<0.05)。[结论]肠梗阻解除后,中药四君子汤可有效地修复损伤的肠黏膜上皮细胞,防止肠道菌群移位,降低血浆内毒素水平,具有保护肠黏膜屏障的功能。[Objective] The changes of intestinal mucosal barrier after relieving obstruction was investigated. And the effects of early ap-plication of Sijunzi decoction on intestinal mucosal barrier after relieving obstruction was assessed. [Methods] The normal rabbits were randomly divided into 6 groups including control group, intestinal obstrution 2 d model group, natural recovery 2 d group, application of Sijunzi decoction 2 d group, natural recovery 2 d group, application of Sijunzi decoction 4 d group. Each group included 6 rabbits. After relieving obstruction the serum endotoxin, Chiu intestinal mucosa injury scores and the integrality of intestinal epithelium was observed by microscope in 2, 4 d. [Results] The level of endotoxin was reduced gradually after relieving obstruction. Moreover, the level of endo-toxin and Chiu’s histopathological evaluation score was obviously lower in the Sijunzi decoction groups than that in the natural recovery groups （P﹤0.05）. The surface area of villus was obviously higher in the Sijunzi decoction groups than that in the natural recovery groups （P﹤0.05）. [Conclusion] The Sijunzi decoction can efficiently restore the lesion of intestinal mucous epithelium to block bacterial translo-cation and to lower the level of endotoxin. Hence, it can obviously protect intestinal mucosal barrier function.

关 键 词：四君子汤 肠黏膜屏障 肠功能障碍 内毒素 肠黏膜损伤评分 

分 类 号：R285.5[医药卫生—中药学]