甜瓜白粉病抗性鉴定方法的比较  

Comparison of Methods for Identifying Disease Resistance to Powdery Mildew

作　　者：齐振宇[1,2] 孔德栋[1] 胡卫珍[1] 金蓉[1] 董正中[1] 孙晓梅[3] 于宁宁[2] 

机构地区：[1]浙江大学农业试验站,杭州310058 [2]浙江大学农业与生物技术学院,杭州310058 [3]上海市农业科学院审计处,上海201403

出　　处：《实验科学与技术》2015年第3期23-26,共4页Experiment Science and Technology

基　　金：浙江大学实验研究基金重点资助项目(188100-560101/024)

摘　　要：采用人工气候箱活体接种、离体叶片圆盘接种两种接种方法对6个甜瓜白粉病病原菌生理小种鉴别寄主进行白粉病接种试验。结果表明,两种方法的试验结果与鉴别寄主抗性相一致,能够准确测定6个甜瓜白粉病病原菌生理小种鉴别寄主对白粉病2F生理小种的感抗性。此外,采用离体叶片圆盘接种法,感病寄主之间病情指数差异不显著,而采用人工气候箱活体接种法,不同感病寄主之间病情指数有显著性差异。The use of artificial climate chamber in vivo and leaf disc method in vitro were inoculated for six species of powdery mildew pathogen identification of physiological host conducted powdery mildew inoculation test. The results of two methods are consistent with the identification of host resistance,and it can accurately distinguish six powdery mildew pathogen differentiating host powdery mildew of physiological races 2F sense resistance. In addition,using in vitro leaf disc inoculation,the difference of disease index between the susceptible hosts was not significant,while the use of artificial climate chamber in vivo inoculation,a significant difference of disease index among susceptible hosts were observed.

关 键 词：白粉病 抗病性鉴定 离体叶片圆盘接种 人工气候箱活体接种 

分 类 号：Q33[生物学—遗传学]