Ⅵ型胶原在正常软骨和骨关节炎软骨中的空间分布  

Changes of distribution of type Ⅵ collagen in osteoarthritis cartilage based on threedimensional images in situ

作　　者：李曌[1,2] 李鹏[1,2] 王恒沙 常童洁[4] 高莹莹[4] 陈超[1] 张里程[1] 张立海[1] 张毅[2] 唐佩福[1] 

机构地区：[1]解放军总医院,北京100853 [2]军事医学科学院基础医学研究所细胞生物学研究室,北京100850 [3]北京雪邦科技有限公司,北京100039 [4]清华大学蛋白质设施细胞影像平台,北京100084

出　　处：《解放军医学院学报》2015年第7期734-737,共4页Academic Journal of Chinese PLA Medical School

摘　　要：目的探究骨关节炎软骨中Ⅵ型胶原空间分布的变化规律。方法应用Hartley豚鼠自发性骨关节炎模型(模型组)和健康豚鼠(对照组),取膝关节软骨做冷冻切片,进行Ⅵ型胶原免疫荧光染色,利用Delta-Vision Elite成像系统和Imaris软件进行断层扫描、3D重建和定量分析,比较两组软骨细胞体积、Ⅵ型胶原厚度与体积的差异。结果正常软骨中,薄层Ⅵ型胶原层均匀包裹软骨细胞,各层细胞外Ⅵ型胶原层厚度无统计学差异(P>0.05),体积随细胞深度增加而增加(P<0.01);骨关节炎软骨中,Ⅵ型胶原体积减小(P<0.01)且空间分布不规则,移行层呈蜂窝状或出现空洞,放射层中呈点、片状弥散分布,且在细胞周基质外出现散在的Ⅵ型胶原。结论在骨关节炎软骨中,Ⅵ型胶原体积减少,不完全包裹软骨细胞,出现蜂窝、空洞和弥散分布的现象,这种改变可能是软骨细胞变性的诱因之一。Objective To explore the changes of spatial distribution of type VI collagen in osteoarthritis cartilage. Methods Frozen sections of full thick cartilage from osteoarthritis and healthy knee of guinea pig in mode group and control group were performed immunofluorescence staining, and then scanned by Delta-Vision Elite system. The scanning data were processed and analyzed by Imaris software, the volume of chondrocyte （Vc）, thickness and volume of type VI collagen （Vp） of the two groups were measured. Results In healthy cartilage, every chondrocyte wore a thin coat of typeⅥ collagen, whose thickness had no significant difference （P 〉 0.05）, and the volume increased （P 〈 0.01） with the increase of depth. In osteoarthritis cartilage, the volume of type VI collagen coat decreased and distributed irregularly. It showed honeycomb distribution and scattered distribution in transition layer and radiation layer, respectively （P 〈 0.01）. Moreover, the scattered type VI collagen was found in extracellular matrix. Conclusion The irregular distribution of type Ⅵcollagen in the osteoarthritis cartilage may be one of the factors which induces chondrocyte degeneration.

关 键 词：Ⅵ型胶原 软骨细胞 骨关节炎 豚鼠自发性骨关节炎模型 

分 类 号：R684.3[医药卫生—骨科学]