不同温室培养条件对栒子微枝试管内生根和成活的影响  

Effects of Different Culture Conditions in Green-House on in Vitro Rooting and Survival of Cotoneaster hjelmqvistii Micro-shoots

作　　者：柴慈江[1] 曹海鹏[1] 王琼[1] 符玲巧[1] 骆建霞[1] 史燕山[1] 

机构地区：[1]天津农学院园艺园林学院,天津300384

出　　处：《天津农学院学报》2015年第2期11-15,共5页Journal of Tianjin Agricultural University

基　　金：国家星火计划项目"优新木本观赏植物组织培养育苗规模化示范"(2008GA610015);天津市星火计划项目"优新木本观赏植物组织培养育苗规模化示范"(08ZHXHNC07000)

摘　　要：为探讨在温室中进行栒子微枝试管内生根的方法和效果,将栒子试管苗茎段接种于灭菌的土壤支撑培养基中,并将培养瓶放入日光温室内,分别在不同遮荫条件下和不同培养时期内进行生根培养,并对温室中试管内生根的栒子试管苗进行气孔开闭状况观察和移栽试验。结果表明,在采用双层遮阳网遮荫的条件下,4月12日至5月22日时期内培养的试管苗生根率达到87.5%,9月2日至10月12日时期内培养的试管苗生根率达到98.1%;温室中培养的栒子试管苗的叶片气孔在黑暗中的关闭率为32.5%,显著高于恒温培养室培养的试管苗(5.0%),表明温室中培养的栒子试管苗在生根过程中已经获得了一定的驯化锻炼;将温室中培养的未经开瓶炼苗的栒子试管苗带坨移入营养钵,在移栽后不覆膜、不喷雾,午后空气相对湿度低至约50%的条件下,移栽成活率达到90%,显著高于恒温培养室培养的试管苗(30.0%)。本结果可进一步降低栒子试管苗的培养成本,并促进栒子组培快繁技术在生产中的应用。s： In order to study the method and effect of Cotoneaster hjelmqvistii micro-shoots rooting in vitro, the stem sections of Cotoneaster hjelmqvistii plantlet in vitro were inoculated in the sterilized medium supported by soil and the cultural bottles were located in green-house, then the rooting culture was completed under different shading conditions or during different culture periods respectively. The opening and closing of stoma was observed and the transplanting experiment was done for the Cotoneaster hjelmqvistii plantlet rooted in vitro in green-house. The results show that shaded by two-story shade net, 87.5% of the stem sections rooted when cultured from April 12 to May 22,and 98.1% of the stem sections rooted when cultured from September 2 to October 12. Under dark conditions, the stoma closing rate of the Cotoneaster hjelmqvistii plantlet cultured in green-house was 32.5%, which was obviously higher than that of the plantlet cultured in the constant temperature room（5.0%）, and this indicated that the plantlet cultured in green-house had been acclimated during the stage of rooting. Without acclimation in opened bottles, the Cotoneaster hjelmqvistii plantlet cultured in green-house were transplanted with lump into the soil, under the conditions without plastic film covering and spraying, and when the relative air humidity was about 50% after noon, the survival rate of the plantlet was 90.0%, obviously higher than that of the plantlet cultured in the constant temperature room （30.3%）. These results can obviously reduce the culture cost of the Cotoneaster hjelmqvistii plantlet in vitro and promote the micro-propagation technology application of Cotoneaster hjelmqvistii to the production.

关 键 词：栒子 微枝 温室 试管内生根 成活率 

分 类 号：S604.3[农业科学—园艺学] S723.132.6