益心泰颗粒对慢性心力衰竭兔Ca^(2+)-CaN-NFAT_3信号通路的影响  

Effects of Yixintai Granule on the Ca^(2+)-CaN-NFAT_3 Signal Pathway in Rabbits with Chronic Heart Failure

作　　者：郭志华[1] 吴刚强[1] 李雅[2] 唐云[1] 毛湘屏[3] 王月爱[1] 孙涛[1] 

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]湖南中医药大学药学院,湖南长沙410208 [3]湖南中医药大学第二附属医院,湖南长沙410005

出　　处：《湖南中医药大学学报》2015年第3期9-11,15,72,共5页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金资助项目(81373816);湖南省研究生科研创新项目(CX2013B327)

摘　　要：目的探讨益心泰颗粒对慢性心力衰竭(chronic heart failure,CHF)兔Ca2+-CaN-NFAT3信号通路的影响。方法用阿霉素建立CHF兔模型,将造模成功兔分为模型组、益心泰低、中、高剂量组和氯沙坦钾组;另设正常对照组。灌胃4周后观察心肌组织病理结构,并检测心肌细胞内钙离子浓度,心肌组织钙调神经磷酸酶(CaN)、T细胞活化因子3(NFAT3m RNA)及其蛋白和磷酸化水平。结果与正常对照组比较,模型组心肌细胞内[Ca2+]i含量、心肌组织CaN水平、NFAT3、p-NFAT3及NFAT3m RNA表达显著升高(P﹤0.01)。与模型组比较,益心泰低剂量组心肌组织NFAT3、NFAT3m RNA表达降低(P﹤0.05),心肌细胞内[Ca2+]i含量、心肌组织CaN水平及p-NFAT3表达显著降低(P﹤0.01),而益心泰中、高剂量组和氯沙坦钾组心肌细胞内[Ca2+]i含量、心肌组织CaN水平、NFAT3、p-NFAT3及NFAT3m RNA表达显著降低(P﹤0.01)。结论益心泰颗粒抑制慢性心力衰竭时Ca2+-CaN-NFAT3信号通路激活,可能是其治疗慢性心衰的主要机制之一。Objective To explore the effects of Yixintai granule on the Ca2＋-CaN-NFAT3 signal pathway in rabbits with chronic heart failure（CHF）. Methods The CHF rabbit models were established with Adriamycin. Then the rabbit models were divided into model group, high-dose, middle-dose, low-dose of Yixintai groups, and Losartan Potassium group. The separate normal group was designed. After 4 weeks of gavage administration, the changes of myocardial tissue pathological structure were observed, and the concentration of myocardial intracellular calciumion, the levels of CaN, NFAT3 m RNA, and its protein and phosphorylation in myocardial tissue were measured. Results Compared with the normal group, the concentration of myocardial intracellular calciumion, the levels of CaN, NFAT3, p-NFAT3, and NFAT3 m RNA were increased significantly（P ﹤ 0.01）. Compared with the model group, the expressions of NFAT3 and NFAT3m RNA in myocardial tissue were decreased（P﹤ 0.05）, the concentration of myocardial intracellular calciumion, the activity of CaN and the expression of p-NFAT3 in myocardial tissue of the low-dose Yixintai group were decreased significantly（P ﹤0.01）; but the concentration of myocardial intracellular calciumion, the levels of CaN, NFAT3, p-NFAT3, NFAT3 m RNA in myocardial tissue of the middle-dose, high-dose of Yixintai group and Losartan Potassium group were decreased significantly（P﹤0.01）. Conclusion The mechanism of Yixintai improving the rabbits with chronic heart failure granule maybe pertinent to inhibiting the Ca2＋-CaN-NFAT3 signal pathway activation.

关 键 词：益心泰 慢性心力衰竭 Ca2+-CaN-NFAT3 

分 类 号：R285.5[医药卫生—中药学] R541[医药卫生—中医学]