“肝病实脾”法对ACLF大鼠内毒素血症的影响  

Effect of "Ganbing Shipi" Method on Endotoxemia in ACLF Rats

作　　者：何琬[1] 陈斌[1] 徐嘉蔚[1] 王国栋[2] 刘璇[2] 

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]湖南中医药大学研究生院,湖南长沙410208

出　　处：《湖南中医药大学学报》2015年第3期12-15,共4页Journal of Hunan University of Chinese Medicine

基　　金：国家中医临床研究基地业务建设科研专项(JDZX2012062);湖南省科技厅科技计划(2013SK3105);湖南省中医药科研基金重点课题(201205)

摘　　要：目的以解毒化瘀法为对照,研究"肝病实脾"法之解毒化瘀、益气健脾法对慢加急性肝衰竭(ACLF)大鼠胃肠动力学及内毒素血症的影响。方法将Wistar大鼠随机分为正常组、模型组、对照组(解毒化瘀)及实验组(解毒化瘀+健脾),采用牛血清白蛋白致敏建立免疫性肝纤维化大鼠模型,然后用D-氨基半乳糖(D-gal)腹腔注射急性攻击建立慢加急性肝衰竭大鼠模型。实验组在牛血清白蛋白攻击的同时用中药健脾颗粒至处死前7 d加用解毒化瘀颗粒进行灌胃治疗,对照组在处死前7 d用解毒化瘀颗粒灌胃治疗进行对照。正常组在腹腔注射生理盐水后处死,其余各组于造模后处死,检测各组大鼠门静脉内毒素、胃动素水平、胃残留率及小肠推进率。结果模型组门静脉内毒素水平较正常组显著升高(P<0.01),胃动素水平下降(P<0.01),胃内残留率显著增加、小肠推进率降低(P<0.01);实验组在造模后观察指标与模型组比较,门静脉内毒素水平降低(P<0.01),胃动素水平升高(P<0.01),胃内残留率显著降低、小肠推进率提高(P<0.01);实验组在造模后各观察指标与对照组比较,门静脉内毒素水平降低(P<0.01),胃动素水平升高(P<0.01),胃内残留率降低(P<0.05)、小肠推进率提高(P<0.01)。结论 ACLF大鼠模型胃动素及胃肠动力存在异常,增强胃肠动力提高胃动素水平,改善肠源性内毒素血症(IETM)是"肝病实脾"法治疗ACLF作用机制之一。Objective To observe the effect of Jiedu Huayu and Yiqi Jianpi from ＂Ganbing Shipi＂ method on gastrointestinal dynamics and endotoxemia in acute-on-chronic liver failure（ACLF） rats, Jiedu Huayu method as the control. Methods The Wistar rats were randomly divided into 4 groups： normal group,model group,control group（Jiedu Huayu granule）,experimental group（Jiedu Huayu and Jianpi granule）. Immunological hepatic fibrosis rat models were induced by administration of bovine serum albumin, and D-galactosamine was simultaneously intra-peritoneally injected to establish the ACLF models. The experiment group was treated with Jiedu Huayu granule at former 7 days in bovine serum albumin attack with Jianpi granule. The control group was intragastrically treated with Jiedu Huayu granule before being killed in 7 days. The rats of normal group were put to death after intraperitoneal injection, and the rest of the group were put them to death after being modeled. Then the level of endotoxin in the portal, motilin, gastric remnant rate and small intestinal propulsion were detected in each group. Results Compared with the normal group, the level of portal endotoxin of model group increased was increased significantly（P〈0.01）, the motilin level was decreased（P〈0.01）, the gastric residual rate increased and the small intestinal propulsion rate was decreased significantly（P〈0.01）. The experiment group compared with the model group, the level of portal endotoxin was decreased（P〈0.01）, motilin level increased（P〈0.01）, gastric emptying rate decreased obviously（P〈0.01）, and small intestinal propulsion rate increased（P〈0.01）. The experiment group compared with the control group, the portal endotoxin was decreased（P〈0.01）, the motilin level was increased（P〈0.01）, the gastric emptying rate was decreased（P〈0.05）, and the small intestinal propulsion rate was increased（P〈0.01）. Conclusion The motilin and gastrointestinal motility in ACLF rat models were abnormal

关 键 词：肝病实脾 慢加急性肝衰竭 内毒素 胃动素 胃排空 小肠推进 

分 类 号：R285.6[医药卫生—中药学] R575.3[医药卫生—中医学]