磁性纳米多孔复合材料的致突变试验研究  

Research of mutagenicity test for magnetic nanometer perforated composite materials

作　　者：黄义鸿[1] 朱伟民[1] 王大平[1] 

机构地区：[1]深圳市第二人民医院风湿免疫科,518035

出　　处：《中国实用医药》2015年第17期36-37,共2页China Practical Medicine

基　　金：深圳市科技研发资金项目(项目编号:CXZZ 20130321152713220);广东省卫生厅医学科研基金项目(项目编号:B2012320);广东省教育厅育才项目(项目编号:2012LYM_0120)

摘　　要：目的从遗传毒理学方面了解并评价磁性纳米多孔复合(Nano-HA/PLLA/Fe2O3)材料的生物相容性,从而为其在移植肌腱固定的临床运用提供依据。方法用Nano-HA/PLLA/Fe2O3磁性纳米复合界面固定材料制备混悬液,进行Ames致突变试验,以检测其对鼠伤寒沙门菌的致突变比值(实验组回变菌落数/阴性对照组回变菌落数)。结果 4个菌株在各个受试浓度待测液在加S9与不加S9的情况下,致突变比值即实验组回变菌落数与阴性对照组回变菌落数的比值(Rt/Rc)均<2.0,而阳性对照组致突变比值则均>2.0。结论 Nano-HA/PLLA/Fe2O3磁性纳米复合界面固定材料不引起鼠伤寒沙门菌的回复突变数增加,说明此材料无致基因突变性。Objective To understand and evaluate the biocompatibility of magnetic nanometer perforated composite materials（Nano-HA/PLLA/Fe2O3） by genetic toxicology, in order to provide reference for its clinical application in tendon transplantion fixation. Methods Suspension was made by Nano-HA/PLLA/Fe2O3 magnetic nanometer composite interface fixation material for Ames mutagenicity test, in order to detect its mutagenicity ratio（number of revertant bacterial colony in research group/number of revertant bacterial colony in negative control group） for salmonella typhimurium. Results All the 4 strains in different tested concentrations with or without S9 had mutagenicity ratio as number of revertant bacterial colony in experimental group/number of revertant bacterial colony in control group（Rt/Rc）〈 2.0, while the ratio with positive control group was 2.0. Conclusion NanoHA/PLLA/Fe2O3 magnetic nanometer composite interface fixation material will not induce increasing number of revertant mutation, which suggests this material contains no mutagenicity.

关 键 词：磁性纳米多孔复合材料 致突变试验 

分 类 号：R318.08[医药卫生—生物医学工程]