阿托伐他汀对自发性高血压大鼠心肌细胞PPAR-γ及GLUT-4表达的影响研究  

Affect of Spontaneously Hypertensive Rat Cardiomyocyte Expression of PPAR-γ and GLUT-4 with Atorvastatin

作　　者：蒲丽君[1] 罗勇[1] 赵珂[1] 

机构地区：[1]川北医学院附属医院,四川南充637000

出　　处：《河北医学》2015年第8期1459-1462,共4页Hebei Medicine

基　　金：川北医学院苗圃基金;(编号:MP-ZK-24)

摘　　要：目的:分析阿托伐他汀对自发性高血压大鼠心肌细胞PPAR-γ及GLUT-4表达水平的影响,为临床运用提供理论指导。方法:选择20只自发性高血压大鼠,随机分为A组(10只)与B组(10只),同时选择10只健康大鼠作为对照组成C组。给予A组大鼠阿托伐他汀按照50mg·kg-1·d-1的水准灌胃喂养,同时给予B组、C组大鼠以同量的蒸馏水灌胃。每隔1周测量三组大鼠的收缩压与舒张压值,测量5次,直到第10周;同时检测三组大鼠10周平均的血脂水平与左心室质量指数水平,比较三组大鼠血脂水平差异性;采用免疫组化法及PT-PCR法检测三组大鼠的PPAR-γ及GLUT-4表达水平。结果:A组与B组大鼠的舒张压与收缩压水平较C组大鼠均较高,具有明显差异性(P<0.05),A组与B组大鼠的血压值水平比较,差异性不明显(P>0.05);A组与B组大鼠的TC、TG、HDLC以及LDL-C等血脂水平较C组大鼠均较低,具有明显差异性(P<0.05),A组与B组比较血脂水平无差异性(P>0.05)。三组大鼠的LVM1水平两两比较均具有显著性差异(P<0.05),但B组大鼠的LVM1水平最高,其次是A组,C组大鼠水平最低;干预前,A组与B组大鼠PPAR-γ及GLUT-4表达水平均较C组较低,具有统计学意义(P<0.05),但A组与B组比较差异性不大(P>0.05);干预后,A组大鼠PPAR-γ及GLUT-4表达水平显著提高,与C组大鼠比较差异性不大,但与B组大鼠比较,具有较大差异性(P<0.05)。结论:阿托伐他汀喂养自发性高血压大鼠,能够降低左心室质量指数水平,但对血压、血脂水平无明显影响,这可能与上调PPAR-γ及GLUT-4表达水平有关,具体机制需要进一步研究。Objective： To analyse affect of spontaneously hypertensive rat cardiomyocyte expression of PPAR-γ and GLUT- 4 with atorvastatin,in order to provide theoretical guidance for clinical application.Method： 20 spontaneously hypertensive rats were randomly divided into group A（ 10） and group B（ 10）,10 healthy rats were selected as group C. Group A were feeded with atorvastatin in dose of 50mg·kg-1·d-1by gavaging,group B and group C were feeded with the same amount of distilled water by gavaging. Systolic and diastolic blood pressure values of the three groups were measured every other week until 10 weeks; Lipid levels and left ventricular mass index of three groups were measured for 10 weeks,Lipid levels of three groups were compared; PPAR-γ and GLUT-4 expression levels of three groups were detected by immunohistochemistry and PT-PCR method. Result： Diastolic and systolic blood pressure levels in group A and group B rats were higher than group C rats（ P〈0.05）,group A and group B median level of blood pressure had no significant（ P〉0.05）; lipid levels of TC,TG,HDL-C and LDL-C in group A and group B were lower than group C rats（ P〈0.05）,lipid levels had no relatively difference between group A and group B（ P〉0.05）.LVM1 levels among three groups were significant differences（ P〈0. 05）,and group B was the highest,group A was Secondly higher,group C was the lowest; before the intervention,PPAR-γ and GLUT- 4 expression of group A and group B was lower than group C（ P〈0.05）,but group A and group B were not significant（ P〉0. 05）; after the intervention,PPAR- γ and GLUT- 4 expression levels was significantly increased,there was not significant compared with group C,but there was significant different compared with group B（ P〈0.05）. Conclusion： Atorvastatin fed spontaneously hypertensive rats,can reduce the level of left ventricular mass index,blood pressure,but no significant effect on blood lipid levels,which may increase PPAR-γ and GLUT-4 expression levels,

关 键 词：阿托伐他汀 自发性高血压大鼠 血压 血脂 PPAR-Γ GLUT-4 

分 类 号：R544.1[医药卫生—心血管疾病]