免疫治疗不明原因反复自然流产患者群体反应抗体、TGF-β和nTreg表达变化的研究  

Alteration of level of panel reactive antibodies,TGF-β in serum and expression rate of nTreg cells in peripheral blood in women with unexplained recurrent spontaneous abortion after immunotherapy

作　　者：任兴斌[1] 张伟[2] 

机构地区：[1]临沂市人民医院检验科,临沂276003 [2]临沂市人民医院创伤骨科,临沂276003

出　　处：《中国免疫学杂志》2015年第6期831-834,共4页Chinese Journal of Immunology

摘　　要：目的:探讨不明原因反复自然流产(URSA)患者经免疫治疗后其血清群体反应抗体(PRA)、转化生长因子β(TGF-β)和外周血单个核细胞(PBMC)中转录调节因子3(FOXP3)阳性的天然调节性T细胞(n Treg,CD4+CD25+FOXP3+)表达的变化及临床意义。方法:应用酶联免疫及流式分析方法,分别检测正常妊娠组及URSA患者免疫治疗后未孕组和妊娠组血清PRA、TGF-β含量及外周血单个核细胞n Treg表达率。结果:RSA患者经免疫治疗后未孕组血清PRA、TGF-β中位数较免疫治疗后妊娠组中位数降低(27.5%、107.7 pg/L vs 68.75%、189.9 pg/L,P<0.01);未孕组n Treg表达率中位数也较妊娠组低(5.05%vs 8.05%,P<0.01)。结论:URSA患者经免疫治疗后其血清PRA的产生及TGF-β含量增加和n Treg的诱导性升高是RSA患者经免疫治疗后成功妊娠的重要因素,PRA、TGF-β及n Treg的检测对RSA患者的免疫治疗方案的确定和疗效判断有重要的临床意义。Objective： To investigate the effects of immunotherapy on PRA,TGF-β and CD4＋CD25＋Foxp3＋n Treg cells in women with unexplained recurrent spontaneous abortion. Methods： We detect the level of PRA,TGF-β in serum and the expression of n Treg cells in peripheral blood of pregnant,non pregnant and natural fertility women by enzyme immunoassay and flow cytometry. Results： After immunotherapy,the median level of PRA and TGF-βin non pregnant group was lower than that in pregnant group（ 27. 5%,107. 7 pg / L vs 68. 75%,189. 9 pg / L,P〈0. 01）. Compared to pregnant group,the median expression rate of n Treg cells were lower in non pregnant group（ 5. 05% vs 8. 05%,P〈0. 01）. Conclusion： The PRA,TGF-β and n Treg cells are critical factors for pregnancy in RSA patients. It is helpful to identify and judge the effect of immunotherapy by detect the level of panel reactive antibodies,TGF-β in serum and the expression rate of n Treg cells in peripheral blood.

关 键 词：免疫治疗 不明原因反复自然流产 转化生长因子-Β 群体反应抗体 天然调节性T细胞 

分 类 号：R392[医药卫生—免疫学]