阿托伐他汀抵抗吸烟相关性血管内皮损伤的实验研究  

Investigation about atorvastatin resist to tobacco smoking inducing endothelial inflammation

作　　者：郭轶[1] 卢小刚[1] 代远斌[2] 

机构地区：[1]重庆市中医院普外科,400021 [2]重庆医科大学附属第一医院血管外科,400016

出　　处：《重庆医学》2015年第19期2616-2617,2620,共3页Chongqing medicine

摘　　要：目的探讨阿托伐他汀对吸烟相关性血管内皮损伤的保护作用。方法将人脐静脉内皮细胞(HUVECs)分为对照组、香烟提取物处理组和阿托伐他汀联合香烟提取物处理组。观察不同组细胞的形态学变化,并检测不同组细胞中血管细胞黏附分子-1(VCAM-1)和E-选择素(E-selectin)的蛋白表达。结果香烟提取物处理组HUVECs的细胞形态发生了剧烈的变化,失去了基本形态;而阿托伐他汀联合香烟提取物处理组HUVECs的细胞形态仅发生了轻微的改变,保留了基本形态。同时VCAM-1和E-selectin蛋白在香烟提取物处理组细胞中表达明显高于对照组和阿托伐他汀联合香烟提取物处理组,差异有统计学意义(P<0.05);VCAM-1和E-selectin蛋白在阿托伐他汀联合香烟提取物处理组细胞中的表达稍高于对照组,差异有统计学意义(P<0.05)。结论阿托伐他汀可以抵抗吸烟导致的血管内皮损伤,具有血管内皮保护作用,可用于治疗吸烟相关性血管疾病。Objective To explore the role of atorvastatin resist to tobacco smoking inducing endothelial inflammation. Methods HUVECs were divided into normal control group,cigarette smoking extract（CSE） group and atorvastatin（AS）＋CSE group. The cellular morphology of HUVECs in three group were observed, then the expressions of VCAM-1 and E selectin in HUVECs in three group were detected by western blot assay. Results In CES group,drastic morphological change of HUVECs were observed. In AS＋CSE group, minor morphological change of HUVECs were observed. Also, the protein levels of VCAM-1 and E selectin were much higher in CSE group than that of in other two groups（P〈0.05）, and the protein levels of VCAM-1 and E-selectin in AS＋CSE group were a little higher than that of in control group,but much lower than that of in CSE group（P〈0.05）. Conclusion Our results showed that atorvastatin might partly resist to tobacco smoking inducing endothelial inflammation.

关 键 词：血管细胞黏附分子-1 E-选择素 吸烟 阿托伐他汀 人脐静脉内皮细胞 

分 类 号：R543[医药卫生—心血管疾病]