GDF9和FST调控猪卵母细胞成熟和胚胎早期发育  

Growth differentiation factor 9 and follistatin regulating porcine oocyte maturation and early embryo development

作　　者：彭中友[1,2] 孙俊铭[3] 李燕[1] 刘庆友[3] 于建宁[1] 施振旦[1] 

机构地区：[1]江苏省农业科学院畜牧研究所/动物品种改良与繁育重点实验室,江苏南京210014 [2]贵州省畜牧兽医学校,贵州贵阳550018 [3]广西大学动物科学学院,广西南宁530004

出　　处：《江苏农业学报》2015年第3期583-589,共7页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省农业科技自主创新基金项目[CX(11)4074];国家科技支撑计划项目(2011BAD19B02-6)

摘　　要：为探讨生长分化因子9(Growth differentiation factor 9,GDF9)和卵泡抑素(Follistatin,FST)如何影响猪卵母细胞成熟及胚胎早期发育能力,在猪卵母细胞体外成熟(IVM)培养过程中添加不同浓度的GDF9和抗GDF9抗体,或同时添加GDF9和FST或抗FST抗体,测定猪卵母细胞的细胞核成熟率、卵裂率、囊胚率及囊胚总细胞数。结果显示,添加GDF9显著提高孤雌胚囊胚率,抗GDF9抗体则显著抑制卵裂率和囊胚率。添加FST显著降低孤雌胚囊胚率和囊胚总细胞数,添加抗FST抗体显著提高了囊胚率。共同添加GDF9与抗FST抗体则使囊胚率达到最高。表明猪卵母细胞IVM期添加GDF9能一定程度上提高卵母细胞及胚胎的发育能力,共同添加GDF9和抗FST抗体对胚胎发育的促进作用具有加性效应。To investigate the effects of growth differentiation factor 9 （GDF9） and follistatin （FST） on porcine oocyte maturation and early embryo developmental competence,different concentrations of exogenous GDF9, anti-GDF9 antibody, FST and anti-FST antibody were added into in vitro maturation medium,and the nuclear maturation, cleavage, blastocyst rate and blastocyst cell number were detected. GDF9 significantly improved blastocyst rate. Anti-GDF9 antibody significantly inhibited cleavage and blastocyst rates. FST addition reduced parthenogenic embryo blastocyst rate and the total cell number of blastocyst. Maturation medium containing anti-FST antibody significantly improved blastocyst rates. Maturation medium containing GDF9 and anti-FST antibody together drove parthenogenic embryo blastocyst rate to the highest level. The results suggested GDF9 addition in maturation medium could improve oocyte maturation and early embryo developmental competence. GDF9 and anti-FST antibody in maturation medium could synergistically improve oocyte developmental competence.

关 键 词：猪 GDF9 抗GDF9抗体 抗FST抗体 囊胚率 卵母细胞发育能力 

分 类 号：S828[农业科学—畜牧学]