补肾、健脾、活血法对骨质疏松症大鼠骨NCX1表达影响比较研究  

Comparative Study of Nourishing-kidney,Strengthening-spleen and Promotingblood Circulation on Expressions of NCX1 in Bone Tissue of Rats with Osteoporosis

作　　者：朱辉[1] 郑洪新[1] 林庶如[1] 杨芳[1] 王剑[1] 张国哲[1] 

机构地区：[1]辽宁中医药大学基础医学院,辽宁沈阳110847

出　　处：《辽宁中医药大学学报》2015年第7期27-30,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：辽宁省科技厅博士启动基金项目(20121102);辽宁省教育厅一般项目(L2012352)

摘　　要：目的:观察中医不同治法对骨质疏松症大鼠骨组织NCX1基因和蛋白表达的影响,探讨骨质疏松症的发病机制及中药治疗的作用机理。方法:将132只Wistar大鼠(雌雄各半)随机分为正常、模型、补肾、健脾、活血及骨疏康组。以地塞米松(2.5 mg/kg,2次/周)肌注造模。除正常组及模型组外,各治疗组予以相应的中药治疗。9周后,测定离体股骨骨密度(BMD)及血清骨代谢相关指标,测定骨组织NCX1 m RNA和蛋白表达。结果:与正常组比较,模型组大鼠BMD降低(P<0.01),血清TRAP含量升高(P<0.01),骨NCX1 m RNA和蛋白表达水平均上升(P<0.01)。与模型组比较,补肾组BMD升高(P<0.01),血清TRAP含量下降(P<0.01),骨NCX1 m RNA和蛋白表达水平下调(P<0.01)。结论:补肾中药可能通过下调骨NCX1表达而抑制破骨细胞骨吸收,从而起到治疗骨质疏松症的作用,其作用优于健脾及活血中药。Objective：To observe the effects of Chinese herbs with different treatment methods on the expressions of NCX1 in bone tissue of rats with osteoporosis. To investigate the pathogenesis of osteoporosis and the treatment mechanism of Chinese herbs. Methods：132 Wistar rats male and female half and half were random Ly divided into normal group,model group,nourishing-kidney group,strengthening-spleen group,activating-blood group and Gushukang group. By intramuscular injection of dexamethasone（2.5 mg·kg-1）twice a week to replicate osteoporosis rat model. To follow with treatment by different Chinese herbs except normal and model groups. After 9 weeks,detected the BMD of femur in vitro and the bone metabolism marker in serum,detected the m RNA and protein expressions of NCX1 in bone tissue. Results：Comparing with the normal group,the BMD of model group decreased（P0.01）,the content of TRAP in serum increased（P0.01）,the m RNA and protein expressions of NCX1 in bone tissue increased. In nourishing-kidney group,the BMD increased（P0.01）,the content of TRAP in serum decreased（P0.01）,the m RNA and protein expressions of NCX1 downregulated comparing with the model group. Conclusion：Nourishing-kidney herbs can inhibit bone resorption of osteoclast through downregulating the expressions of NCX1 in bone tissue,so can have the effect of anti-osteoporosis,the treatment effect have an advantage compared to strengthening-spleen and promoting-blood circulation herbs.

关 键 词：骨质疏松症 补肾 健脾 活血 钠钙交换体亚型1 

分 类 号：R274[医药卫生—中医骨伤科学]