IL-1β和IL-2的表达在大鼠心律失常发生中作用  

Effect of the Expression of IL-1β and IL-2 in the Role of Arrhythmia in Rats

作　　者：孙大明[1,2] 昌艳艳[3] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110847 [2]朝阳市中心医院,辽宁朝阳122000 [3]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《辽宁中医药大学学报》2015年第7期33-35,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：辽宁省科学技术计划项目(2011225040)

摘　　要：目的:研究探讨IL-1β和IL-2与心律失常的关系,及其在表达过程中对心律失常的影响和作用。方法:72只大鼠随机分成6组每组12只,即对比组、模型组、空白治疗组、西药组、中药组、中西药结合组,采用分别灌注IL-1β和IL-2的方法,观察大鼠心律变化,通过动态监测IL-1β与IL-2对大鼠血浆神经肽的影响及各个时段的心电图变化,同时利用Western Blot和激光聚焦(LSCM)显微镜检测IL-1β和IL-2的蛋白表达水平。结果:灌注IL-1β组的大鼠心肌组织表达空白组和西药组表达显著增加(P<0.05),灌注IL-2组的大鼠心肌组织表达空白组、中西药结合组表达显著增加(P<0.05)。结论:灌注IL-1β和IL-2后的大鼠心肌组织发生变化,大量的IL-1β和IL-2可能对大鼠心律失常的形成有诱导或促进作用。Objective：To study the IL-1 beta and IL-2 and the relationship between cardiac arrhythmia, and its effect on cardiac arrhythmias in the process of expression and function. Methods：72 rats were divided into 6 groups at random,each group of 12 blank control group,model group,treatment group,western medicine,Chinese medicine group,the combination of Chinese and western group,using respectively perfusion IL-1 beta and IL-2, the method of theory method in the rat heart rhythm changes,through dynamic monitoring IL-1 beta and IL-2 of the effect of rat plasma neuropeptides and electrocardiogram（ecg） changes every time, at the same time using the Western Blot and laser focusing microscope（LSCM）detection of IL-1 beta and IL-2 protein expression levels. Results：Perfusion IL-1 beta rat myocardial tissue expression of empty group and combination of Chinese and western group increased significantly（P0.05）,perfusion IL-2 groups of dazu county impatient expression empty group,Chinese medicine group,the combination of Chinese and western expression increased significantly（P0.05）. Conclusion：Perfusion IL-1 beta and IL-2 after the rats myocardial tissue changes,a large number of IL-1 beta and IL-2 May induce or promote the formation of the rats cardiac arrhythmias of role.

关 键 词：心律失常 心电技术 大鼠 

分 类 号：R541.7[医药卫生—心血管疾病]