同种异体Sprague-Dawley大鼠子宫内膜异位症动物模型的建立  

Establishment of endometriosis model in allogeneic Sprague-Dawley rats

作　　者：崔金秀[1] 顾振鹏[1] 刘志慧[1] 李芳芳[1] 

机构地区：[1]山东省滨州医学院附属医院,256600

出　　处：《中华妇幼临床医学杂志（电子版）》2015年第3期94-97,共4页Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)

摘　　要：目的探讨采用皮下移植法建立大鼠同种异体子宫内膜异位症（EMS）模型的可行性。方法选择健康、未交配且处于动情期（正常动情周期≥2个）的雌性Sprague-Dawley（SD）大鼠48只为研究对象。按照随机数字表法,将48只SD大鼠平均分为供鼠（n=24）和受鼠（n=24）。于SD大鼠处于动情期时将供鼠子宫组织缝合于受鼠腹部皮下,并于手术后3周取出异位病灶组织,于光学显微镜下观察异位病灶组织的形态学及组织病理学结果。结果 48只SD大鼠均有规律的4个动情周期,分别为动情前期、动情期、动情后期、动情间期,各个周期分别为4～5d。24只SD大鼠（受鼠）均存活,同种异体SD大鼠皮下移植法成模率为66.7%（16/24）,其中16只SD大鼠腹部肉眼可见球型凸起,触之呈囊实性,无活动性,切开皮肤可见透亮的球型囊状增生物,质地软,囊内有清亮液体聚积,平均体积为（34.5±19.8）mm3,异位病灶组织于光学显微镜低倍镜（×100）下可见异位病灶生长成一个腔样结构,于高倍镜下（×400）可见内膜上皮细胞、间质细胞及少量腺体形成,组织病理学表现与人EMS相似。结论通过皮下移植法建立的同种异体SD大鼠EMS模型的异位病灶组织的组织病理学改变与人EMS类似,可以作为EMS研究的动物模型。Objective To explore the feasibility of the establishment of endometriosis（EMS）model in allogeneic rats by abdominal subcutaneous implantation.Methods Choose 48 healthy,unmated and in estrus（normal estrous cycle ≥ 2）female Sprague-Dawley（SD）rats as the research object.According to random number table,48 SD rats were divided into donor rats（n=24）and recipient rats（n=24）.When the SD rats in estrus,we sutured the donor rats uterine tissue to the abdominal subcutaneous of recipient rats,and removed the ectopic lesion tissue 3 weeks after surgery,then observe histopathologic findings under an optical microscope.Results All of those 48 SD rats had regular estrous cycles,there were4 estrous cycles which were proestrus,estrus,metestrus,and diestrus,each period lasted 4-5d.Twentyfour SD rats（recipient rats） were alive,the successful rate of allogeneic abdominal subcutaneous implantation of SD rats was 66.7%（16/24）,in which 16 SD rats′abdomen could see dome projection,which was cystic and solid,non-active,and cut the skin open,we can see translucent dome cystic proliferator,texture soft,clear liquid accumulation in the capsule.The average volume was（34.5±19.8）mm3.Ectopic lesion tissue was observed through low magnification optical microscope（×100）,and we could see ectopic lesions grew into a cavity-like structure.Through we could see endometrial epithelial cells,stromal cells and the formation of a small amount of glandular under high magnification optical microscope（×400）.Tissue pathology was similar to human EMS.Conclusions The tissue pathology changes of ectopic endometrial in EMS model of allogeneic SD rats by subcutaneous transplantation were similar to human EMS,EMS SD rats model can be used in the studies on EMS.

关 键 词：子宫内膜异位症 模型 动物 移植 同种 大鼠 

分 类 号：R711.71[医药卫生—妇产科学] R-332[医药卫生—临床医学]