辛伐他汀对缺血再灌注损伤下内皮祖细胞凋亡的影响  

Effects of Simvastatin on apoptosis of endothelial progenitor cells against ischemia-reperfusion injury

作　　者：李上海[1] 梁伟钧[1] 王苗[1] 

机构地区：[1]广东医学院附属医院心内科,广东湛江524000

出　　处：《中国医药导报》2015年第19期11-14,共4页China Medical Herald

基　　金：广东省科技计划项目(2011B031800325)

摘　　要：目的观察辛伐他汀对缺血再灌注损伤下内皮祖细胞(EPCs)凋亡的影响。方法将EPCs随机分为对照组(C组)、缺血再灌注组(IR组)、辛伐他汀组(S组),MTT法测定各组EPCs增殖情况,流式细胞术测定各组EPCs凋亡率。结果与C组比较,IR组EPCs抑制率上升[(18.00±0.00)%比(3.60±0.33)%],细胞凋亡率上升[(9.55±0.79)%比(4.68±0.33)%],差异有统计学意义(P<0.01、P<0.05);而S组EPCs增殖率上升[(53.00±0.00)%比(13.38±1.80)%],凋亡率下降[(2.42±0.37)%比(4.68±0.33)%],差异有统计学意义(P<0.05)。结论辛伐他汀通过促进EPCs增殖能力、减少EPCs凋亡来发挥其对缺血再灌注损伤下EPCs的保护作用。Objective To observe the effects of Simvastatin on apoptosis of endothelial progenitor cells against ischemi-a-reperfusion injury. Methods EPCs were randomly divided into control group （group C）, ischemia-reperfusion group （group IR）, Simvastatin group （group S）. The proliferation of EPCs was detected by MTT assay. At the same time, the apoptosis rates of EPCs were detected by flow cytometry. Results Compared with group C, the inhibition of EPCs in group IR increased [（18.00±0.00）%v s （3.60±0.33）%], the apoptotic rate increased clearly [（9.55±0.79）%v s （4.68±0.33）%], the differences were statistically significant （P〈0.01, P〈0.05）; however, the proliferation ratio of EPCs in group S in-creased [（53.00±0.00）% vs （13.38±1.80）%], while the apoptotic rate decreased [（2.42±0.37）% vs （4.68±0.33）%], the dif-ferences were statistically significant （P 〈 0.05）. Conclusion Simvastatin protects EPCs in the ischemia-reperfusion environment by promoting proliferation capacity and reducing apoptosis rates.

关 键 词：辛伐他汀 缺血再灌注损伤 内皮祖细胞 细胞凋亡 

分 类 号：R965[医药卫生—药理学]