HPLC法测定复方土牛膝咽喉合剂中12,13-二羟基泽兰素的含量  

Determination of 12,13-dihydroxyeuparin in Complex Radix Eupatorii Chinensis Mixture by HPLC

作　　者：罗新中[1] 傅玉新[2] 陈莉[2] 曾文谊[2] 刘碧波[2] 

机构地区：[1]广东医学院附属石龙博爱医院,东莞523326 [2]东莞市人民医院,东莞523059

出　　处：《北方药学》2015年第7期2-3,共2页Journal of North Pharmacy

基　　金：东莞市科技计划医疗卫生类科研一般项目(项目编号:2014105101180)

摘　　要：目的：建立高效液相色谱法测定复方土牛膝咽喉合剂中12,13-二羟基泽兰素含量的方法,为复方土牛膝咽喉合剂的质量控制提供依据。方法：采用反相高效液相色谱法,色谱柱为Kromasil C18柱（250mm×4.6mm,5μm）,流动相为乙腈-0.1%磷酸水溶液梯度洗脱,流速为1m L·min-1,检测波长为240nm,柱温为30℃。结果：12,13-二羟基泽兰素含量测定的线性范围0.00414～0.02484mg·m L-1,r2=0.9945,平均加样回收率为95.87%,RSD为2.60%。结论：建立测定复方土牛膝咽喉合剂中12,13-二羟基泽兰素含量的高效液相色谱法,该方法准确、可靠,可用于复方土牛膝咽喉合剂的质量评价。Objective To establish a method for the content determination of 12, 13-dihydroxyeuparin in Complex Radix Eupatorii Chinensis Mixture by HPLC.Methods HPLC was developed to determine 12, 13-dihydroxyeuparin with UV absorptions at 240nm. The analysis was performed on a Kromasil C18 column （250mm×4.6mm,5μm）with a gradient solvent system of acetonitrile and 0.1%aqueous phosphoric acid at a flow rate of 1.0mL·min-1. Results There was a good linear relationship （r2=0.9945）in the range of 0.00414～0.02484mg·mL-1. The mean recovery was 95.87%, RSD was 2.60%（n=6）. Conclusion This method is simple,sensitive and accurate,and can be used for quality control of Complex Radix Eupatorii Chinensis Mixture.

关 键 词：土牛膝合剂12 13-二羟基泽兰素高效液相色谱法 

分 类 号：R284[医药卫生—中药学]