基质辅助激光解吸电离飞行时间质谱在临床病原菌鉴定中的应用  

Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for the identification of clinical isolates

作　　者：王卫萍[1] 奚海燕[1] 黄梅[1] 王洁[1] 范明[1] 陈勇[1] 邵海枫[1] 

机构地区：[1]南京军区南京总医院解放军临床检验医学研究所,南京210002

出　　处：《临床检验杂志》2015年第5期337-340,共4页Chinese Journal of Clinical Laboratory Science

基　　金：全军医学科技"十二五"科研项目(CWS129811298);南京军区医学科技创新课题(10M101)

摘　　要：目的探讨基质辅助激光解吸电离飞行时间质谱（matrix-assisted laser desorption ionization-time of flight mass spectrometry,MALDI-TOF MS）系统用于快速鉴定临床分离菌的可靠性和实用性。方法收集南京军区南京总医院2013年7~10月自临床标本分离的非重复细菌1 061株,分别使用MALDI-TOF MS和Vitek 2 Compact全自动细菌鉴定仪系统进行鉴定,结果不一致的菌株采用16S r DNA测序验证。结果 1 061株临床分离菌中1 058株（99.7%）经MALDI-TOF MS系统正确鉴定到属水平,1 016株（95.8%）正确鉴定到种,其余3株（0.3%）未给出鉴定结果。5株鉴定结果不一致的菌株经16S r DNA测序确认,结果与MALDI-TOF MS和Vitek 2 Compact鉴定符合率分别为40.0%（2/5）和0（0/5）。结论 MALDI-TOF MS可以作为一个快速、准确和价廉的工具应用于临床分离菌的鉴定。Objective To evaluate the clinical performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry（ MALDI-TOF MS） system in the identification of clinical isolates. Methods A total of 1 061 non-repetitive isolates were collected from clinical culture samples in our hospital during Jul 2013 to Oct 2013,and identified by MALDI-TOF MS and Vitek 2 Compact system,respectively. The conflicting results between them were further verified by 16 S r DNA gene sequencing. Results Among 1 061 clinical isolates,1 058（ 99. 7%） were identified at genus level by MALDI-TOF MS system,and the other 3（ 0. 3%） were not.Among 1 058 isolates identified by MALDI-TOF MS system,1 016（ 95. 8%） were identified at species level. Five isolates with conflicting results between MALDI-TOF MS and Vitek 2 Compact systems were verified by 16 S r DNA gene sequencing,and the coincidence rates with the former and the latter were 40. 0%（ 2 /5） and 0（ 0 /5）,respectively. Conclusion The MALDI-TOF MS system may be used to identify clinical isolates rapidly,accurately,and inexpensively.

关 键 词：基质辅助激光解吸电离飞行时间质谱 临床菌株 快速鉴定 

分 类 号：R446.5[医药卫生—诊断学]